Induction of de novo synthesis of phenylalanine ammonia-lyase by L-alpha-aminooxy-beta-phenylpropionic acid in suspension cultures of Daucus carota L

• Published in: Planta Vol. 154; no. 5; pp. 454 - 458
• Main Authors: Noe, W, Seitz, H.U
• Format: Journal Article
• Language: English
• Published: Springer-Verlag 01-01-1982
• Subjects: Antiserum; carrots; Cell culture techniques; Chemical suspensions; Cultured cells; Electrophoresis; Enzymes; Gels; Immunoprecipitation; Parsley; Physiological regulation
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/BF01267813

Application of L-α-aminooxy-β-phenylpropionic acid (L-AOPP), a potent and specific competitive inhibitor of L-phenylalanine ammonia-lyase (PAL), to an anthocyanin-producing cell suspension culture of Daucus carota results in a dramatic increase in extractable PAL activity and an accumulation of phenylalanine (Noé et al., 1980, Planta 149, 283—287). Using an immunoprecipitation technique, evidence was obtained that the increase in PAL activity the result of de-novo synthesis. The activity of the other enzymes of the general phenylpropanoid metabolism, e.g., trans-cinnamate 4-hydroxylase and hydroxycinnamate: CoA ligase, were not affected by L-AOPP. This result strongly supports the view that PAL is regulated independently.