Resistance of Initiation Factor 2 (eIF-2α) Kinases to Staurosporine: An Approach for Assaying the Kinases in Crude Extracts

We studied the effect of staurosporine on two well characterised mammalian eIF-2α kinases, the heme-regulated translational inhibitor (HRI), and interferon-inducible double-stranded RNA-activated protein kinase (PKR). Both pure eIF-2 and a synthetic peptide used to measure the activity of purified o...

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Bibliographic Details
Published in:	Cellular signalling Vol. 11; no. 6; pp. 399 - 404
Main Authors:	Martı́n de la Vega, C, Garcı́a, A, Martı́n, M.E, Alcázar, A, Marin, O, Quevedo, C, Salinas, M
Format:	Journal Article
Language:	English
Published:	England Elsevier Inc 01-06-1999
Subjects:	Animals eIF-2 Kinase - metabolism eIF-2α Kinase Eukaryotic initiation factor eIF-2 Neocortex - enzymology Peptide phosphorylation Rats Staurosporine Tissue Extracts Translational control eIF-2α Kinase Peptide phosphorylation Eukaryotic initiation factor eIF-2 Staurosporine IFN–interferon PI–phosphatase inhibitors Translational control HRI–heme-regulated translational inhibitor, (alternatively referred to as HCR, heme-controlled repressor, or HCI, heme-controlled inhibitor) dsRNA–double-stranded RNA poly(I·C) or pIC–poly(I):poly(C) eIF-2α– the α subunit of eIF-2 eIF-2–eukaryotic initiation factor 2 PKR–double-stranded RNA activated protein kinase (also called p68 or DAI) PMS–postmitochondrial supernatant 2 mM β-glycerophosphate, 2 mM sodium molybdate and 10 mM NaF
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