Cloning and sequences of inducible and constitutive macrolide resistance genes in Staphylococcus aureus that correspond to an ABC transporter

• Published in: FEMS microbiology letters Vol. 181; no. 1; pp. 91 - 100
• Main Authors: Matsuoka, Mayumi, Jánosi, László, Endou, Kikutarou, Nakajima, Yoshinori
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-12-1999
• Subjects: ABC transporter; Active efflux; Erythromycin resistance; macrolide antibiotics; Macrolide resistance; macrolides; msr gene; plasmid pEP2104; plasmid pMC38; plasmid pMR504; Staphylococcus aureus; streptogramin B; msr gene; Macrolide resistance; Erythromycin resistance; Active efflux; ABC transporter; Staphylococcus aureus
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/S0378-1097(99)00518-2

A restriction map was made and the DNA sequence was determined for a plasmid, pMC38, derived from the inducible macrolide resistance plasmid pEP2104, that showed constitutive resistance to PMS antibiotics (partial macrolide and streptogramin B antibiotics). A 5.04 kb
SalI-
PstI fragment (fragment C) of pMC38, which encoded PMS resistance, was cloned into a shuttle vector, pRIT5, to yield pMR504. The transformant
Staphylococcus aureus 4220 (pMR504) exhibited constitutive PMS resistance. Fragment C was subcloned to pUC19 in order to determine the DNA sequence. This sequence was consequently found to contain three open reading frames (ORF1–3), of which ORF3 corresponded to the 63 kDa membrane protein (MsrSA) that expressed PMS resistance. According to DNA sequence comparison of the control region of ORF3 in pMC38 and pEP2104, 44 nucleotides including RBS1 and the leader peptide (MTASMRLK) were deleted on plasmid pMC38. This suggests that the leader peptide is essential for the inducible expression of PMS resistance.