Stabilization of homogeneous preparations of pregnancy zone protein lyophilized in the presence of saccharose: Structural and functional studies

Human pregnancy zone protein (PZP) is a macromolecule of 360 kDa, organized as a disulfide-linked homodimer of two 180 kDa subunits, with an amino acid sequence and structure remarkably similar to that of human α2-Macroglobulin. Homogeneous PZP samples undergo fast aging forming oligomeric aggregate...

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Bibliographic Details
Published in:	Journal of biochemical and biophysical methods Vol. 46; no. 1; pp. 95 - 105
Main Authors:	Bonacci, Gustavo, Sánchez, Marı́a C, Gonzalez, Martı́n, Ceschin, Danilo, Fidelio, Gerardo, Vides, Miguel.A, Chiabrando, Gustavo
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 20-11-2000
Subjects:	Calorimetry, Differential Scanning Chymotrypsin - metabolism Dimerization Electrophoresis, Polyacrylamide Gel Endopeptidases - metabolism Female Freeze Drying Freezing Humans Low Density Lipoprotein Receptor-Related Protein-1 Pregnancy Proteins - chemistry Pregnancy Proteins - metabolism Pregnancy zone protein, Protein stability, Saccharose Preservation, Biological - methods Protein Binding Protein Conformation Protein Denaturation Receptors, Immunologic - metabolism Sucrose - metabolism Thermodynamics Trehalose - metabolism α2-Macroglobulin α2-Macroglobulin Pregnancy zone protein, Protein stability, Saccharose
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