Chemical composition and phytotoxic activity of clove (Syzygium aromaticum) essential oil obtained with supercritical CO2

• Published in: The Journal of supercritical fluids Vol. 118; pp. 185 - 193
• Main Authors: de Oliveira, Mozaniel Santana, da Costa, Wanessa Almeida, Pereira, Daniel Santiago, Botelho, José Rafael Santos, de Alencar Menezes, Tatiany Oliveira, de Aguiar Andrade, Eloisa Helena, da Silva, Silvia Helena Marques, da Silva Sousa Filho, Antonio Pedro, de Carvalho, Raul Nunes
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-12-2016
• Subjects: Allelopathy; Hypocotyl; Inhibition; Radicle; Seed; Supercritical fluid; Weed; Hypocotyl; Weed; Seed; Allelopathy; Supercritical fluid; Inhibition; Radicle
• ISSN: 0896-8446; 1872-8162
• DOI: 10.1016/j.supflu.2016.08.010

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⿢Different fractions of clove essential oil were obtained with supercritical CO2.⿢The highest mass yield was achieved at 50°C and 300bar.⿢The species M. pudica had the greater inhibition of seed germination.⿢The radicle and hypocotyl of S. obtusifolia were more resistant to the phytotoxic effects.⿢Eugenol proved to be the main phytotoxic activity promoter.
Clove (S. aromaticum) essential oils were obtained with supercritical CO2 in the experimental conditions of 40°C and 50°C and pressures of 100, 200, and 300bar. The oils were characterized by gas chromatography (GC) coupled to a mass spectrometer (MS). The phytotoxic effects of the fractions initially at 600ppm were analyzed. From the results observed in the phytotoxic effect analysis, the fraction obtained in the condition of 50°C and 300bar was analyzed at concentration levels ranging from 200 to 800ppm. The highest mass yield in dry basis (db) (14.49±0.2%) was obtained at 50°C and 300bar. Over 99% of the volatile compounds were identified in clove essential oil, comprising 24 substances. In global means, 19.72% of the compounds are composed of sesquiterpene hydrocarbons, 19.79% of phenolic compounds, 0.59% of oxygenated sesquiterpenes, 59.75% of oxygenated monoterpenes, and 0.055% of other compounds. All fractions showed phytotoxic activity to inhibit seed germination and elongation of the radicle and hypocotyl. The best results were shown by the extract obtained at 50°C and 300bar. The results showed that eugenol plays an active role as a phytotoxic-activity-promoting agent. In terms of minimum concentration to inhibit 50% and 90% of seed germination and elongation of the radicle and hypocotyl, the extract obtained at 50°C and 300bar was the most effective.