The role of the nAChR subunits α5, β2, and β4 on synaptic transmission in the mouse superior cervical ganglion

• Published in: Physiological reports Vol. 7; no. 6; pp. e14023 - n/a
• Main Authors: Simeone, Xenia, Karch, Rudolf, Ciuraszkiewicz, Anna, Orr‐Urtreger, Avi, Lemmens‐Gruber, Rosa, Scholze, Petra, Huck, Sigismund
• Format: Journal Article
• Language: English
• Published: United States John Wiley and Sons Inc 01-03-2019; Wiley
• Subjects: Animals; Cells, Cultured; Compound action potential; EPSP; Ganglionic Blockers - pharmacology; Hexamethonium; knockout mice; Mice, Inbred C57BL; Mice, Knockout; Nerve Tissue Proteins - deficiency; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Neurons - drug effects; Neurons - metabolism; Neuroscience; nicotinic ACh receptor; Nicotinic Antagonists - pharmacology; Original Research; Receptors, Nicotinic - deficiency; Receptors, Nicotinic - genetics; Receptors, Nicotinic - metabolism; Signalling Pathways; superior cervical ganglion; Superior Cervical Ganglion - drug effects; Superior Cervical Ganglion - metabolism; Synaptic Potentials; synaptic transmission; Synaptic Transmission - drug effects; nicotinic ACh receptor; knockout mice; EPSP; superior cervical ganglion; synaptic transmission; Compound action potential; Hexamethonium
• ISSN: 2051-817X
• DOI: 10.14814/phy2.14023

Our previous immunoprecipitation analysis of nicotinic acetylcholine receptors (nAChRs) in the mouse superior cervical ganglion (SCG) revealed that approximately 55%, 24%, and 21% of receptors are comprised of α3β4, α3β4α5, and α3β4β2 subunits, respectively. Moreover, mice lacking β4 subunits do not express α5‐containing receptors but still express a small number of α3β2 receptors. Here, we investigated how synaptic transmission is affected in the SCG of α5β4‐KO and α5β2‐KO mice. Using an ex vivo SCG preparation, we stimulated the preganglionic cervical sympathetic trunk and measured compound action potentials (CAPs) in the postganglionic internal carotid nerve. We found that CAP amplitude was unaffected in α5β4‐KO and α5β2‐KO ganglia, whereas the stimulation threshold for eliciting CAPs was significantly higher in α5β4‐KO ganglia. Moreover, intracellular recordings in SCG neurons revealed no difference in EPSP amplitude. We also found that the ganglionic blocking agent hexamethonium was the most potent in α5β4‐KO ganglia (IC50: 22.1 μmol/L), followed by α5β2‐KO (IC50: 126.7 μmol/L) and WT ganglia (IC50: 389.2 μmol/L). Based on these data, we estimated an IC50 of 568.6 μmol/L for a receptor population consisting solely of α3β4α5 receptors; and we estimated that α3β4α5 receptors comprise 72% of nAChRs expressed in the mouse SCG. Similarly, by measuring the effects of hexamethonium on ACh‐induced currents in cultured SCG neurons, we found that α3β4α5 receptors comprise 63% of nAChRs. Thus, in contrast to our results obtained using immunoprecipitation, these data indicate that the majority of receptors at the cell surface of SCG neurons consist of α3β4α5.
We analyzed synaptic transmission in the superior cervical ganglion of wild‐type mice and mice with deletions of distinct nAChR subunit genes. By measuring the inhibition of hexamethonium in α5β2‐KO, and by modeling its effect on a population of “pure” α3β4α5 receptors, we estimated that α3β4α5 receptors comprise 72% of nAChRs expressed on the mouse SCG plasma membrane. This figure is three times higher than the number of solubilized receptors determined by immunoprecipitation.