In-vitro Study of Photothermal Anticancer Activity of Carboxylated Multiwalled Carbon Nanotubes

• Published in: Journal of biomedical physics and engineering Vol. 7; no. 4; pp. 317 - 332
• Main Authors: Bahreyni-Toosi, M H, Zare, M H, Ale-Davood, A, Shakeri, M T, Soudmand, S
• Format: Journal Article
• Language: English
• Published: Iran Journal of Biomedical Physics and Engineering 01-12-2017; Shiraz University of Medical Sciences
• Subjects: Hyperthermia; Multiwalled Carbon Nanotubes; Nanoparticles; Near Infrared; Original; Photothermal Therapy; Nanoparticles; Photothermal Therapy; Multiwalled Carbon Nanotubes; Near Infrared; Hyperthermia
• ISSN: 2251-7200; 2251-7200
• DOI: 10.22086/jbpe.v0i0.367

Multi-walled Carbon Nano Tubes (MWCNTs) as an important element of nanosciences have a remarkable absorption in the region of NIR window (650-900 nm) which can overcome the limitations of deep treatment in photothermal therapy. To disperse MWCNTs in water, it is proposed to attach carboxylated functional group (-COOH) to MWCNTs in order to increase dispersivity in water.
A stable suspension of MWCNTs-COOH with different concentrations (from 2.5 to 500 μg/ml) was prepared. Then, they were compared for their ability to increase temperature in the presence of 810 nm laser irradiation and through a wide range of radiation time (from 20 to 600 s) and three laser powers (1.5, 2 and 2.5 w). The temperature rise was recorded real time every 20 seconds by a precise thermometer.
Absorption spectrum of MWCNTs-COOH suspension was remarkably higher than water in a wavelength range of 200 to 1100 nm. For example, using the concentrations of 2.5 and 80 μg/ml of MWCNTs-COOH suspension caused a temperature elevation 2.35 and 9.23 times compared to water, respectively, upon 10 min laser irradiation and 2.5 w. Moreover, this predominance can be observed for 1.5 and 2 w radiation powers, too. Our findings show that the maximum of temperature increase was obtained at 80 μg/ml concentration of MWCNT-COOH suspension for three powers and through all periods of exposure time. Our results show that the minimum required parameters for a 5°C temperature increase (a 5°C temperature increase causes cell death) were achieved through 2.5 w, 28 μg/ml concentration and 20 second irradiation time in which both concentration and radiation times were relatively low.
Our results showed that MWCNTs-COOH can be considered as a potent photothermal agent in targeted therapies. New strategies must be developed to minimize the concentration, irradiation time and radiation power used in experiments.