Next-generation sequencing for lung cancer

• Published in: Zhongguo fei ai za zhi Vol. 17; no. 1; pp. C1 - C12
• Main Authors: Wu, Kehua, Huang, R Stephanie, House, Larry, Cho, William Chi
• Format: Journal Article
• Language: Chinese
• Published: China Chinese Anti-Cancer Association Chinese Antituberculosis Association 01-01-2014; Chinese Anti-Cancer Association; Chinese Antituberculosis Association
• Subjects: Consortia; Deoxyribonucleic acid; DNA; DNA methylation; Epidermal growth factor; Exons - genetics; Gene expression; Genetic testing; Genomes; Genomics; High-Throughput Nucleotide Sequencing - methods; Humans; Kinases; Lung cancer; Lung neoplasms; Lung Neoplasms - drug therapy; Lung Neoplasms - genetics; Molecular Targeted Therapy; Mutation; Patients; Precision medicine; Sequence Analysis, RNA; United States > US
• ISSN: 1009-3419; 1999-6187; 1999-6187
• DOI: 10.3779/j.issn.1009-3419.2014.01.10

肺癌在生物学上具有侵袭性，并且是癌症相关死亡的主要原因。根据临床特征、预后、对治疗的反应和耐受性，每一例肺癌患者的进展均是独特的。传统上基于毛细管的单基因测序的第一代技术（如Sanger测序法）已被允许大量平行测序且成本更低、通量更高的下一代测序技术（next-generation sequencing, NGS）所替代。与传统方法相比，NGS技术取得显著进步。我们希望这些方法可全面地解释癌症全球图谱，并提供更多信息以满足个体化用药的需求。本综述包括对不同NGS技术的简要说明，NGS在肺癌研究进展中的应用和重要发现的总结，包括对已知靶基因（EGFR、ALK和KRAS）的进一步探索、其它肺癌突变的鉴定和癌症基因组研究的全局协调。