CHARACTERISTICS OF ADHESION AND PROLIFERATION OF MOUSE NIH/3T3 FIBROBLASTS ON THE POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE) FILMS WITH DIFFERENT SURFACE ROUGHNESS VALUES

• Published in: Vestnik transplantologii i iskusstvennykh organov Vol. 14; no. 1; pp. 72 - 77
• Main Authors: V.А. Surguchenko, А.S. Ponomareva, А.Е. Efimov, Е.А. Nemets, I.I. Agapov, V.I. Sevastianov
• Format: Journal Article
• Language: Russian
• Published: Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov 01-03-2012
• Subjects: broblasts, proliferation, metabolic activity, bacterial copolymer, roughness
• DOI: 10.15825/1995-1191-2012-1-72-77
• ISSN: 1995-1191

Adhesion and proliferation of NIH/3Т3 mouse fibroblasts on the surfaces of bacterial copolymer poly(3-hydroxy- butyrate-co-3-hydroxyvalerate) films with different roughness was investigated. Atomic force microscopic analysis showed that surface roughness values of all films were significantly greater (92.0 ± 7.0; 290.8 ± 7.0; 588.8 ± 16.0 nm) than that of the cultural plastic control (9.5 ±0.6 nm). It was revealed that adhesion and metabolic activity of the cells decreases with the increase of surface roughness values. NIH/3Т3 mouse fibroblasts attached weakly to the surface of copolymer films and washed away the substrate during rinsing and staining.