Detection of Brucella by Peripheral Blood PCR and Comparison with Culture and Serological Methods in Suspected Cases

• Published in: Iranian journal of public health Vol. 37; no. 4; pp. 96 - 102
• Main Authors: B Kazemi, SA Yousefi Namin, M Bandepour, F Kafilzadeh, L Gachkar, F Mahmoudinejad, A Samarghandi, M Mardani
• Format: Journal Article
• Language: English
• Published: Tehran University of Medical Sciences 01-12-2008
• Subjects: Brucella; Culture and serological methods; PCR
• ISSN: 2251-6085

"nBackground: Brucellosis is a zoonotic disease and its symptoms and appearances are not exclusive in human and its tradi­tional diagnosis is based on blood culture and serological methods. For more sensitive and specific detection, the PCR method is recommended."nMethods: One hundred four blood samples were gathered from suspicious patients with brucellosis different ages form Ker­manshah, Mazandaran, Khorassan and Hormozgan provinces, and were examined by Rose Bengal, anti globulin, culture and PCR methods."nResults: Seventy three samples were positive by PCR method, 15 samples were positive by cultured method and 84 sam­ples were positive by serological methods."nConclusion: PCR method is sensitive and specific for diagnosis of Brucella from peripheral blood in suspected cases.