Producción de Ácidos Grasos Poliinsaturados a partir de Biomasa Microalgal en un Cultivo Heterotrófico

• Published in: Ion (Bucaramanga, Colombia) Vol. 30; no. 1; pp. 91 - 103
• Main Authors: Medina, Gloria Inés Leal, Bonett, José Eduardo Abril, Gélvez, Silvia Juliana Martínez, Peñaloza, Yaneth Amparo Muñoz, Lizarazo, Elena María Peñaranda, Suárez, Néstor Andrés Urbina
• Format: Journal Article
• Language: English; Portuguese; Spanish
• Published: Bucaramanga Universidad Industrial de Santander, Escuela de Ingeniería Química 01-06-2017; Universidad Industrial de Santander
• Subjects: Arachidonic acid; bomasa microalgal; Chlorella sp; Cultivation; ENGINEERING, CHEMICAL; Fatty acids; Gas chromatography; Lipids; Nitrogen; Oleic acid; Palmitic acid; Productivity; Reaction kinetics; Scenedesmus; Scenedesmus sp; Stearic acid; ácidos grasos poliinsaturados (PUFA’s); biomassa de microalgas; ácidos graxos poliinsaturados (PUFAs); Chlorella sp; Scenedesmus sp; bomasa microalgal; microalgal biomass; PUFA’s; ácidos grasos poliinsaturados (PUFA’s)
• ISSN: 0120-100X; 2145-8480
• DOI: 10.18273/revion.v30n1-2017007

In this research, we obtained Polyunsaturated Fatty Acids (PUFAs) from Microalgal Biomass in heterotrophic culture. In order to obtain these PUFA's we used Chlorella sp. and Scenedesmus sp. strains with heterotrophic conditions. Subsequently, we selected the strain with higher productivity and determined the growth kinetics and biomass, glucose, phosphorus and nitrogen yields and the lipids extracted analyzed by gas chromatography. The heterotrophic culture was established in 1L Continuos Stirred-Tank Reactor (CSTR) under specific conditions; 28 degees Celsius, 1vvm, pH 6,8 and a relation C/N 12:1. Then, we performed sub-cultivation in a "Bioreactor BioFlo115" with 10L volume and made productivity analysis of lipids. Lipids profile allowed us to determine that higher Fatty Acid present in Chlorella sp. is oleic acid (C 18:1) and represented a percentage of 28,75 of total fatty acids. Also we noted the accumulation the other fatty acids like; palmitoleic acid (C 16:1) 19.75%, arachidonic acid (C 20:0) 19.37%, linoleic acid (C 18:2) 11.86%, palmitic acid (C 16:0) 7.24%, linoleic acid (...-C 18:3), 2.61%, erucic acid (C 22:1) 4.61%, stearic acid (C 18:0) 2.4%. (ProQuest: ... denotes formulae/symbols omitted.)