A Three-Step Approach to Estimation of Reduction Potentials of Natural Mixtures of Antioxidants Based on DPPH Test; Illustration for Catechins and Cocoa

• Published in: Proceedings Vol. 11; no. 1; p. 4
• Main Authors: Barbara Kusznierewicz, Monika Baranowska, Klaudia Suliborska, Wojciech Chrzanowski, Agnieszka Bartoszek
• Format: Journal Article
• Language: English
• Published: MDPI AG 01-04-2019
• Subjects: antioxidants; DPPH test; standard reduction potential; stoichiometry value
• ISSN: 2504-3900
• DOI: 10.3390/proceedings2019011004

The aim of this study is to propose a methodology to assess electrochemical properties of complex mixtures of antioxidants, such as plant extracts, based on the results of simple and popular DPPH test. The first, most difficult step, involves determinations of standard reduction potentials (E0) for the series of purified compounds (here catechins). The next step is the calculation of stoichiometric values (n10) based on the results of DPPH test for the same compounds. Finally, a correlation equation is formulated, which is then employed to estimate “cumulative reduction potential” (Ec) for the mixture of interest (here cocoa) using DPPH test results.