LC/MS/MS analysis of α-tocopherol and coenzyme Q 10 content in lyophilized royal jelly, beebread and drone homogenate

• Published in: Journal of mass spectrometry. Vol. 51; no. 11; pp. 1023 - 1029
• Main Authors: Hryniewicka, Marta, Karpinska, Agnieszka, Kijewska, Marta, Turkowicz, Monika Joanna, Karpinska, Joanna
• Format: Journal Article
• Language: English
• Published: England 01-11-2016
• Subjects: alpha-Tocopherol - analysis; Animals; Bees - chemistry; Chromatography, High Pressure Liquid; Fatty Acids - chemistry; Food Analysis; Humans; Nutrition Assessment; Propolis - chemistry; Tandem Mass Spectrometry; Ubiquinone - analogs & derivatives; Ubiquinone - analysis; coenzyme Q10; beebread; LC-MS analysis; drone homogenate; royal jelly; α-tocopherol
• ISSN: 1076-5174; 1096-9888
• DOI: 10.1002/jms.3821

This study shows the results of application liquid chromatography-tandem mass spectrometry (LC/MS/MS) for assay of the content of α-tocopherol and coenzyme Q
in bee products of animal origin, i.e. royal jelly, beebread and drone homogenate. The biological matrix was removed using extraction with n-hexane. It was found that drone homogenate is a rich source of coenzyme Q
. It contains only 8 ± 1 µg/g of α-tocopherol and 20 ± 2 µg/g of coenzyme Q
. The contents of assayed compounds in royal jelly were 16 ± 3 and 8 ± 0.2 µg/g of α-tocopherol and coenzyme Q
, respectively. Beebread appeared to be the richest of α-tocopherol. Its level was 80 ± 30 µg/g, while the level of coenzyme Q
was only 11.5 ± 0.3 µg/g. Copyright © 2016 John Wiley & Sons, Ltd.