Cytoprotective Effects of Lysophospholipids from Sea Cucumber Holothuria atra

• Published in: PloS one Vol. 10; no. 8; p. e0135701
• Main Authors: Nishikawa, Yoshifumi, Furukawa, Ayumi, Shiga, Ikumi, Muroi, Yoshikage, Ishii, Toshiaki, Hongo, Yayoi, Takahashi, Shunya, Sugawara, Tatsuya, Koshino, Hiroyuki, Ohnishi, Masao
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 14-08-2015; Public Library of Science (PLoS)
• Subjects: Animals; Antioxidants; Apoptosis; Apoptosis - drug effects; Biosynthesis; Cell Line; Chemical compounds; Chromatography; Cytoprotection - drug effects; Gene expression; Holothuria - chemistry; Holothuria atra; Holothuroidea; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; Invertebrates; Laboratories; Lipids; Liquid chromatography; Lysophosphatidylcholine; Lysophospholipids - chemistry; Lysophospholipids - pharmacology; Macrophages; Macrophages - metabolism; Magnetic resonance; Marine invertebrates; Mass spectrometry; Metabolites; Mice; Molecular chains; NMR; Nuclear magnetic resonance; Oxidative stress; Oxidative Stress - drug effects; Oxygen; Pharmacology; Physiological aspects; Platelets; Reactive oxygen species; Signal transduction; Spectrometry; Sulfuric acid; Veterinary medicine; Japan; Germany; Okinawa
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0135701

Lysophospholipids are important signaling molecules in animals and metazoan cells. They are widely distributed among marine invertebrates, where their physiological roles are unknown. Sea cucumbers produce unique lysophospholipids. In this study, two lysophospholipids were detected in Holothuria atra for the first time, lyso-platelet activating factor and lysophosphatidylcholine, with nuclear magnetic resonance and liquid chromatography-time-of-flight mass spectrometric analyses. The lipid fraction of H. atra contained lyso-platelet activating factor and lysophosphatidylcholine, and inhibited H2O2-induced apoptosis in the macrophage cell line J774A.1. The antioxidant activity of the lysophospholipid-containing lipid fraction of H. atra was confirmed with the oxygen radical absorbance capacity method. Our results suggest that the lysophospholipids from H. atra are potential therapeutic agents for the inflammation induced by oxidative stress.