New technologies for DNA analysis – a review of the READNA Project
•Discovery of ‘TUF’ DNA – unusually difficult to denature stretches of genomic DNA sequence.•Enrichment methods – new methods, benchmarking and descriptors of enrichment technologies.•Manipulating, visualizing and interrogating individual DNA molecules in nanosystems.•Nanopore Sequencing – first com...
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| Published in: | New biotechnology Vol. 33; no. 3; pp. 311 - 330 |
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| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
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Elsevier B.V
25-05-2016
Elsevier |
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| Abstract | •Discovery of ‘TUF’ DNA – unusually difficult to denature stretches of genomic DNA sequence.•Enrichment methods – new methods, benchmarking and descriptors of enrichment technologies.•Manipulating, visualizing and interrogating individual DNA molecules in nanosystems.•Nanopore Sequencing – first commercial nanopore sequencer from Oxford Nanopore Technologies.
The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 41/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3rd and 4th generation of sequencing methods with nanopores and in situ sequencing, respectively. |
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| AbstractList | The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received 12 million s funding under the European Union Framework Programme 7 from 1st June 2008 to 30th November 2012. The 19 project partners from both academia and industry from in total 7 countries had a project budget of 16 Ms with which they have discovered, created and developed a huge body of insights into nucleic acid analysis. Results have been presented widely in publications and in innumerous public presentations. Results have been moved to spin-offs such as the Olink enrichment kits (now sold by Agilent as Haloplex) and are findingtheir way to the market, such as the Oxford Nanopore MinIon sequencer that was first released to early-access user sites in 2014. The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 41/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3(rd) and 4(th) generation of sequencing methods with nanopores and in situ sequencing, respectively. The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 4 1/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3rd and 4th generation of sequencing methods with nanopores and in situ sequencing, respectively. The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 4 1/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3(rd) and 4(th) generation of sequencing methods with nanopores and in situ sequencing, respectively. The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 4 1/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3 super(rd) and 4 super(th) generation of sequencing methods with nanopores and in situ sequencing, respectively. •Discovery of ‘TUF’ DNA – unusually difficult to denature stretches of genomic DNA sequence.•Enrichment methods – new methods, benchmarking and descriptors of enrichment technologies.•Manipulating, visualizing and interrogating individual DNA molecules in nanosystems.•Nanopore Sequencing – first commercial nanopore sequencer from Oxford Nanopore Technologies. The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 41/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3rd and 4th generation of sequencing methods with nanopores and in situ sequencing, respectively. |
| Author | Brookes, Anthony J. Hohlbein, Johannes Maglia, Giovanni Oostmeijer, Jelle Lehrach, Hans Stähler, Peer F. Out, Ruud Picaud, Vincent Gut, Marta Le Reste, Ludovic van Helvoort, Joop M.L.M. Evans, Geraint Persson, Fredrik Stade, Björn Mir, Kalim Willcocks, James P. Schracke, Nadine McGinn, Steven Kristensen, Anders Veal, Colin D. Freitag, Camilla Dong, Liqin Ekström, Björn Franke, Andre Schyns, Emile Brown, Tom van der Zaag, Pieter J. Heath-Brun, Isabelle El-Sagheer, Afaf Fritzsche, Joachim Sauer, Sascha Brefort, Thomas Moens, Lotte Stoddart, David Bauer, David Elsharawy, Abdou Gullberg, Mats Tegenfeldt, Jonas O. Gut, Ivo G. Teng, Xia Falk-Sörqvist, Elin Kapanidis, Achillefs N. Flyvbjerg, Henrik Heron, Andrew J. Ke, Rongqin Mertes, Florian Beier, Markus Nilsson, Mats Rotem, Dvir Fredriksson, Simon Dekker, Cees Pedersen, Jonas Nyvold Lancaster, Owen Guenther, Simone Sengenes, Jennifer Bayley, Hagan Gibson, Spencer Mangion, Jonathan Freeman, Peter Tost, Jörg Forster, Michael Mignardi, Marco Kaye, Jane Marie, Rodolphe Heath, Simon Zahra, Nathalie Mauger, Fl |
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| Issue | 3 |
| Keywords | signal processing, bioinformatics, statistical analysis, Nucleic Acid analysis, classification |
| Language | English |
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| Snippet | •Discovery of ‘TUF’ DNA – unusually difficult to denature stretches of genomic DNA sequence.•Enrichment methods – new methods, benchmarking and descriptors of... The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 41/2 years. The... The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 4 1/2 years. The... The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received 12 million s funding under the European Union Framework Programme... |
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| Title | New technologies for DNA analysis – a review of the READNA Project |
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