Direct and dynamic detection of HIV-1 in living cells

In basic and applied HIV research, reliable detection of viral components is crucial to monitor progression of infection. While it is routine to detect structural viral proteins in vitro for diagnostic purposes, it previously remained impossible to directly and dynamically visualize HIV in living ce...

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Bibliographic Details
Published in:	PloS one Vol. 7; no. 11; p. e50026
Main Authors:	Helma, Jonas, Schmidthals, Katrin, Lux, Vanda, Nüske, Stefan, Scholz, Armin M, Kräusslich, Hans-Georg, Rothbauer, Ulrich, Leonhardt, Heinrich
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 28-11-2012 Public Library of Science (PLoS)
Subjects:	Amino Acid Sequence Antibodies Antibody Affinity - immunology Antigens Biology Biosensors Capsid protein Cell Membrane - metabolism Cells (biology) Chromatography Detection equipment Development and progression Diagnostic systems Fluorescence gag Gene Products, Human Immunodeficiency Virus - immunology gag Gene Products, Human Immunodeficiency Virus - metabolism Gag protein Genetic modification Genetically modified organisms Health aspects HeLa Cells HIV HIV Core Protein p24 - chemistry HIV Core Protein p24 - immunology HIV Core Protein p24 - metabolism HIV tests HIV-1 - immunology HIV-1 - isolation & purification HIV-1 - metabolism Human immunodeficiency virus Humans Image analysis Image processing Immunoglobulins Infection Infectious diseases Kinetics Light microscopy Medicine Microscopy Microscopy, Confocal Molecular Imaging Molecular Sequence Data Morphogenesis Nanobodies Protein binding Protein Binding - immunology Protein Transport Proteins Recovery (Medical) Single-Domain Antibodies - immunology Single-Domain Antibodies - metabolism Time-Lapse Imaging Veterinary medicine Viral proteins Virions Virology Virus Assembly Viruses Germany
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