The expression and function of fatty acid transport protein-2 and -4 in the murine placenta

• Published in: PloS one Vol. 6; no. 10; p. e25865
• Main Authors: Mishima, Takuya, Miner, Jeffrey H, Morizane, Mayumi, Stahl, Andreas, Sadovsky, Yoel
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 20-10-2011; Public Library of Science (PLoS)
• Subjects: Amnion; Animals; Automation; Biology; Blood circulation; Breastfeeding & lactation; Cell Hypoxia; Coenzyme A Ligases - genetics; Coenzyme A Ligases - metabolism; Embryogenesis; Embryonic growth stage; Embryos; Fatty Acid Transport Proteins - genetics; Fatty Acid Transport Proteins - metabolism; Fatty acids; Female; Fetal development; Fetuses; Gene expression; Gene Expression Regulation; Gynecology; Humans; Hypoxia; Lipids; Medicine; Mice; Mutation; Obstetrics; Placenta; Placenta - cytology; Placenta - metabolism; Polymerase chain reaction; Pregnancy; Protein transport; Proteins; Transport; Transport proteins; Triglycerides; Trophoblasts; Trophoblasts - cytology; Trophoblasts - metabolism; United States > US; Pittsburgh Pennsylvania; Pennsylvania
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0025865

The uptake and trans-placental trafficking of fatty acids from the maternal blood into the fetal circulation are essential for embryonic development, and involve several families of proteins. Fatty acid transport proteins (FATPs) uniquely transport fatty acids into cells. We surmised that placental FATPs are germane for fetal growth, and are regulated during hypoxic stress, which is associated with reduced fat supply to the fetus.
Using cultured primary term human trophoblasts we found that FATP2, FATP4 and FATP6 were highly expressed in trophoblasts. Hypoxia enhanced the expression of trophoblastic FATP2 and reduced the expression of FATP4, with no change in FATP6. We also found that Fatp2 and Fatp4 are expressed in the mouse amnion and placenta, respectively. Mice deficient in Fatp2 or Fatp4 did not deviate from normal Mendelian distribution, with both embryos and placentas exhibiting normal weight and morphology, triglyceride content, and expression of genes related to fatty acid mobilization.
We conclude that even though hypoxia regulates the expression of FATP2 and FATP4 in human trophoblasts, mouse Fatp2 and Fatp4 are not essential for intrauterine fetal growth.