Comparison of a Flow Assay for Brucellosis Antibodies with the Reference cELISA Test in West African Bos indicus

• Published in: PloS one Vol. 4; no. 4; p. e5221
• Main Authors: Bronsvoort, Barend M. DeC, Koterwas, Bronwyn, Land, Fiona, Handel, Ian G, Tucker, James, Morgan, Kenton L, Tanya, Vincent N, Abdoel, Theresia H, Smits, Henk L
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 20-04-2009; Public Library of Science (PLoS)
• Subjects: Animals; Antibodies; antibody detection; antigen detection; Antigens; Bayes Theorem; Bayesian analysis; Biomedical research; Bos indicus; Bovidae; Brucella; Brucellosis; Brucellosis - immunology; Brucellosis - veterinary; Bubalus bubalis; Cattle; cattle diseases; Cattle Diseases - immunology; Competition; competitive enzyme linked immunosorbent assay; Developing countries; Diagnostic software; Diagnostic systems; Diagnostic tests; Disease; Disease control; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzymes; Epidemiology; Health aspects; Immunoassay; immunoassays; Immunoglobulins; Immunology; Infections; Infectious Diseases/Epidemiology and Control of Infectious Diseases; Laboratories; lateral flow assay; LDCs; Livestock; Mathematics/Statistics; Measles; Mumps; Population; Public health; Public Health and Epidemiology/Epidemiology; Rubella; serodiagnosis; Studies; Veterinary medicine; Viral antibodies; Yersinia; Yersinia enterocolitica; zebu; Zoonoses; Cameroon; Netherlands
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0005221

Brucellosis is considered by the Food and Agricultural Organisation and the World Health Organisation as one of the most widespread zoonoses in the world. It is a major veterinary public health challenge as animals are almost exclusively the source of infection for people. It is often undiagnosed in both human patients and the animal sources and it is widely acknowledged that the epidemiology of brucellosis in humans and animals is poorly understood, particularly in sub-Saharan Africa. It is therefore important to develop better diagnostic tools in order to improve our understanding of the epidemiology and also for use in the field for disease control and eradication. As with any new diagnostic test, it is essential that it is validated in as many populations as possible in order to characterise its performance and improve the interpretation of its results. This paper describes a comparison between a new lateral flow assasy (LFA) for bovine brucellosis and the widely used cELISA in a no gold standard analysis to estimate test performance in this West African cattle population. A Bayesian formulation of the Hui-Walter latent class model incorporated previous studies' data on sensitivity and specificity of the cELISA. The results indicate that the new LFA is very sensitive (approximately 87%) and highly specific (approximately 97%). The analysis also suggests that the current cut-off of the cELSIA may not be optimal for this cattle population but alternative cut-offs did not significantly change the estimates of the LFA. This study demonstrates the potential usefulness of this simple to use test in field based surveillance and control which could be easily adopted for use in developing countries with only basic laboratory facilities.