Characterization and Expression of Turkey Prolactin Regulatory Element Binding in the Anterior Pituitary Gland and Pancreas During Embryogenesis
The PRL regulatory element-binding (PREB) protein is a transcription factor that was originally cloned from the rat anterior pituitary gland and characterized as a regulator of the PRL promoter. It is also strongly expressed in several extrapituitary tissues; however, its functional role is not well...
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| Published in: | The Journal of Poultry Science Vol. 53; no. 1; pp. 67 - 75 |
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Japan Poultry Science Association
01-01-2016
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| Abstract | The PRL regulatory element-binding (PREB) protein is a transcription factor that was originally cloned from the rat anterior pituitary gland and characterized as a regulator of the PRL promoter. It is also strongly expressed in several extrapituitary tissues; however, its functional role is not well understood to date. In this study, we aimed to clone and characterize the turkey PREB gene and investigate its mRNA expression in the anterior pituitary gland and pancreas during embryogenesis. Based on the conserved sequence of chicken and mammalian PREB cDNAs, a turkey PREB cDNA fragment was obtained, and after sequencing of the fragment, the 5′- and 3′-ends of mRNA were amplified and determined. To identify the PREB gene structure, polymerase chain reaction (PCR) amplification was performed. The turkey PREB gene consists of 9 exons and 8 introns, and it encodes a 411-amino-acid protein. The expression of PREB mRNA in the anterior pituitary gland was measured during embryogenesis. Levels of PREB mRNA significantly increased at embryonic day 22, with maximum levels being detected on day 25 of ontogeny, which correlated with similar changes in levels of PRL mRNA. The highest level of PREB mRNA was detected on day 19 in the pancreas. However, the highest level of insulin mRNA was detected at embryonic day 25. These results indicate that PREB may be involved in the expression of PRL mRNA in the anterior pituitary gland, whereas insulin mRNA may be expressed independently of the expression of PREB mRNA in the pancreas during embryogenesis. |
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| AbstractList | The PRL regulatory element-binding (PREB) protein is a transcription factor that was originally cloned from the rat anterior pituitary gland and characterized as a regulator of the PRL promoter. It is also strongly expressed in several extrapituitary tissues; however, its functional role is not well understood to date. In this study, we aimed to clone and characterize the turkey PREB gene and investigate its mRNA expression in the anterior pituitary gland and pancreas during embryogenesis. Based on the conserved sequence of chicken and mammalian PREB cDNAs, a turkey PREB cDNA fragment was obtained, and after sequencing of the fragment, the 5′- and 3′-ends of mRNA were amplified and determined. To identify the PREB gene structure, polymerase chain reaction (PCR) amplification was performed. The turkey PREB gene consists of 9 exons and 8 introns, and it encodes a 411-amino-acid protein. The expression of PREB mRNA in the anterior pituitary gland was measured during embryogenesis. Levels of PREB mRNA significantly increased at embryonic day 22, with maximum levels being detected on day 25 of ontogeny, which correlated with similar changes in levels of PRL mRNA. The highest level of PREB mRNA was detected on day 19 in the pancreas. However, the highest level of insulin mRNA was detected at embryonic day 25. These results indicate that PREB may be involved in the expression of PRL mRNA in the anterior pituitary gland, whereas insulin mRNA may be expressed independently of the expression of PREB mRNA in the pancreas during embryogenesis. |
| Author | Hiyama, Gen Zadworny, David Wakui, Shin McQuaid, Rosanne Kansaku, Norio |
| Author_xml | – sequence: 1 fullname: Hiyama, Gen organization: Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, Japan – sequence: 2 fullname: Kansaku, Norio organization: Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, Japan – sequence: 3 fullname: Wakui, Shin organization: Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, Japan – sequence: 4 fullname: McQuaid, Rosanne organization: Department of Animal Science, McGill University, Canada – sequence: 5 fullname: Zadworny, David organization: Department of Animal Science, McGill University, Canada |
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| Cites_doi | 10.1159/000054502 10.1073/pnas.74.12.5463 10.1016/S0014-5793(98)01168-5 10.1095/biolreprod64.5.1575 10.1007/s003350010142 10.2141/jpsa.0140036 10.1126/science.257.5073.1115 10.1111/j.1582-4934.2008.00469.x 10.1016/0016-6480(89)90102-0 10.1016/0016-6480(91)90153-W 10.1016/0012-1606(89)90237-6 10.1006/gcen.2000.7507 10.2141/jpsa.46.6 10.2307/1538937 10.1038/nmeth.2089 10.1095/biolreprod61.3.757 10.1007/s00125-006-0255-y 10.1016/j.ygcen.2004.11.017 10.1038/347528a0 10.1210/mend.13.4.0260 10.1677/joe.0.1220005 10.1002/jmor.1050880104 10.1016/j.ygcen.2004.05.012 10.1016/0016-6480(86)90057-2 10.1016/S0378-1119(03)00804-7 10.1038/263211a0 10.1210/mend-3-10-1529 10.1126/science.1157704 10.2141/jpsa.45.1 |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 The nucleotide sequences reported in this paper have been submitted to GenBank/EMBL/DDBJ Bank with the accession number LC036259 (turkey PREB cDNA) and LC036258 (turkey PREB gene). |
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| Title | Characterization and Expression of Turkey Prolactin Regulatory Element Binding in the Anterior Pituitary Gland and Pancreas During Embryogenesis |
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