A Genetic Linkage Map for the Zebrafish

• Published in: Science (American Association for the Advancement of Science) Vol. 264; no. 5159; pp. 699 - 703
• Main Authors: Postlethwait, John H., Johnson, Stephen L., Midson, Clare N., Talbot, William S., Gates, Michael, Ballinger, Eric W., Africa, Dana, Andrews, Rebecca, Carl, Tim, Eisen, Judith S., Horne, Sally, Kimmel, Charles B., Hutchinson, Mark, Johnson, Michele, Rodriguez, Andre
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 29-04-1994; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Alleles; Animals; Biological and medical sciences; Chromosome Mapping; Classical genetics, quantitative genetics, hybrids; Cloning, Molecular; Danio; Danio rerio; DNA; DNA mapping; Embryos; Female; Fish; Freshwater; Fundamental and applied biological sciences. Psychology; Genetic aspects; Genetic loci; Genetic mapping; Genetic Markers; Genetic mutation; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Genomes; Genotype; Haploidy; Male; Mutation; Neurology; Phenotype; Polymerase Chain Reaction; Repetitive Sequences, Nucleic Acid; Software; Vertebrata; Vertebrates; Zebra fish; Zebrafish; Zebrafish - genetics; Genetic mapping; Vertebrata; Brachydanio rerio; Genome organization; Linkage; Gene; Random amplified polymorphic DNA; Pisces; Chromosome; Mutation
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.8171321

To facilitate molecular genetic analysis of vertebrate development, haploid genetics was used to construct a recombination map for the zebrafish Danio (Brachydanio) rerio. The map consists of 401 random amplified polymorphic DNAs (RAPDs) and 13 simple sequence repeats spaced at an average interval of 5.8 centimorgans. Strategies that exploit the advantages of haploid genetics and RAPD markers were developed that quickly mapped lethal and visible mutations and that placed cloned genes on the map. This map is useful for the position-based cloning of mutant genes, the characterization of chromosome rearrangements, and the investigation of evolution in vertebrate genomes.