Effect of nitrogenous resource on growth, biochemical composition and ultrastructure of I sochrysis galbana ( I sochrysidales, H aptophyta)
Summary The nitrogenous resource used to promote algal growth has cost implications for mass culture processes. The present study therefore aimed to determine the effect of different nitrogenous resources (nitrate, ammonium and urea) on various performance parameters (growth, final cell yield, pigme...
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| Published in: | Phycological research Vol. 63; no. 1; pp. 43 - 50 |
|---|---|
| Main Authors: | , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
01-01-2015
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| Abstract | Summary
The nitrogenous resource used to promote algal growth has cost implications for mass culture processes. The present study therefore aimed to determine the effect of different nitrogenous resources (nitrate, ammonium and urea) on various performance parameters (growth, final cell yield, pigmentation, lipid yield and cellular and sub‐cellular characteristics) in
I
sochrysis galbana
. Growth rate was unaffected by nitrogenous resource, but the final cellular yield in the nitrate and urea treatments far exceeded that evident in the ammonium treatments. The reduced cell yield in ammonium treatments and the earlier onset of the stationary phase was brought about by nitrogen‐starvation due to an increase in
pH
and resultant ammonia volatilization. This starvation initiated an early onset of lipid accumulation, chlorophyll depletion and an increase in the carotenoid to chlorophyll ratio relative to the other nitrogen (
N
) source treatments. Hence, in spite of being potentially the preferred source of
N
by algae (due to its reduced state), ammonium‐nitrogen is undesirable for mass culture. The performance parameters of
I
sochrysis
grown in urea (an organic
N
source) and nitrate (an inorganic
N
source) were similar, but lipid accrued earlier in cells grown in medium supplemented with urea. This is advantageous for lipid acquisition for the production of biodiesel since it would reduce the duration of photobioreactor runs. Urea is easily available and considerably cheaper than all the other
N
sources tested and is thus recommended as the nitrogenous resource for large‐scale culture of
I
. galbana
for biodiesel production. |
|---|---|
| AbstractList | Summary
The nitrogenous resource used to promote algal growth has cost implications for mass culture processes. The present study therefore aimed to determine the effect of different nitrogenous resources (nitrate, ammonium and urea) on various performance parameters (growth, final cell yield, pigmentation, lipid yield and cellular and sub‐cellular characteristics) in
I
sochrysis galbana
. Growth rate was unaffected by nitrogenous resource, but the final cellular yield in the nitrate and urea treatments far exceeded that evident in the ammonium treatments. The reduced cell yield in ammonium treatments and the earlier onset of the stationary phase was brought about by nitrogen‐starvation due to an increase in
pH
and resultant ammonia volatilization. This starvation initiated an early onset of lipid accumulation, chlorophyll depletion and an increase in the carotenoid to chlorophyll ratio relative to the other nitrogen (
N
) source treatments. Hence, in spite of being potentially the preferred source of
N
by algae (due to its reduced state), ammonium‐nitrogen is undesirable for mass culture. The performance parameters of
I
sochrysis
grown in urea (an organic
N
source) and nitrate (an inorganic
N
source) were similar, but lipid accrued earlier in cells grown in medium supplemented with urea. This is advantageous for lipid acquisition for the production of biodiesel since it would reduce the duration of photobioreactor runs. Urea is easily available and considerably cheaper than all the other
N
sources tested and is thus recommended as the nitrogenous resource for large‐scale culture of
I
. galbana
for biodiesel production. |
| Author | Roopnarain, Ashira Sym, Stuart Gray, Vincent Myles |
| Author_xml | – sequence: 1 givenname: Ashira surname: Roopnarain fullname: Roopnarain, Ashira organization: School of Molecular and Cell Biology University of the Witwatersrand Johannesburg Gauteng South Africa – sequence: 2 givenname: Stuart surname: Sym fullname: Sym, Stuart organization: School of Animal, Plant and Environmental Science University of the Witwatersrand Johannesburg Gauteng South Africa – sequence: 3 givenname: Vincent Myles surname: Gray fullname: Gray, Vincent Myles organization: School of Molecular and Cell Biology University of the Witwatersrand Johannesburg Gauteng South Africa |
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| CitedBy_id | crossref_primary_10_3390_fermentation10040220 crossref_primary_10_1016_j_csr_2021_104506 crossref_primary_10_3390_su15054210 crossref_primary_10_4490_algae_2016_31_8_18 crossref_primary_10_3390_w14020221 crossref_primary_10_1016_j_fuel_2019_115789 crossref_primary_10_1007_s10811_019_02015_0 crossref_primary_10_1111_pre_12099 crossref_primary_10_1021_acs_iecr_9b02134 crossref_primary_10_3390_f13010079 |
| Cites_doi | 10.1016/j.biortech.2011.04.006 10.1016/j.jphotobiol.2005.11.002 10.1038/265373a0 10.2134/jeq1974.00472425000300020003x 10.1016/j.biortech.2012.12.159 10.1016/0022-0981(72)90041-X 10.1016/j.biombioe.2012.03.010 10.1111/j.0022-3646.1990.00393.x 10.1111/j.1399-3054.1982.tb00352.x 10.1111/j.1529-8817.2012.01154.x 10.1016/0144-4565(86)90011-9 10.1016/S0044-8486(96)01416-0 10.4319/lo.1972.17.2.0265 10.1080/09670269300650071 10.1111/j.1399-3054.1971.tb01445.x 10.1016/0044-8486(94)90125-2 10.1023/A:1012537219198 10.1111/j.1529-8817.2012.01177.x 10.1080/09670262.2011.554886 10.1021/ac60111a017 10.1111/pre.12054 10.1046/j.1469-8137.1999.00539.x 10.1016/0043-1354(76)90154-8 10.4319/lo.1989.34.6.1014 10.2216/i0031-8884-36-5-345.1 10.1007/s12010-008-8443-5 10.1080/00103627509366547 10.1016/0960-8524(91)90142-7 10.1111/j.0022-3646.1993.00587.x 10.1007/BF02442457 10.1016/S0022-5320(69)90033-1 10.1023/A:1011925022941 10.1016/j.procbio.2005.03.006 10.1093/plankt/12.2.381 10.1016/j.biortech.2010.06.036 10.1023/A:1007921728300 10.1016/j.apenergy.2012.03.027 10.1016/B0-12-176480-X/00353-3 10.2216/i0031-8884-41-2-158.1 10.1016/B978-0-12-372522-6.00007-4 10.1016/j.phytochem.2011.12.007 10.1016/0044-8486(91)90279-G 10.1139/m62-029 10.1023/A:1026007902078 10.1007/BF01313275 10.1002/bit.260240308 10.1016/S0044-8486(98)00278-6 |
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| References | e_1_2_7_5_1 e_1_2_7_3_1 e_1_2_7_9_1 e_1_2_7_7_1 e_1_2_7_19_1 e_1_2_7_17_1 e_1_2_7_15_1 e_1_2_7_41_1 e_1_2_7_13_1 e_1_2_7_43_1 e_1_2_7_11_1 e_1_2_7_45_1 e_1_2_7_47_1 e_1_2_7_26_1 e_1_2_7_49_1 e_1_2_7_28_1 Syrett P. J. (e_1_2_7_50_1) 1988; 28 e_1_2_7_25_1 e_1_2_7_31_1 e_1_2_7_52_1 e_1_2_7_23_1 e_1_2_7_33_1 e_1_2_7_35_1 e_1_2_7_37_1 e_1_2_7_39_1 e_1_2_7_6_1 e_1_2_7_8_1 e_1_2_7_18_1 e_1_2_7_16_1 e_1_2_7_40_1 e_1_2_7_2_1 Golterman H. L. (e_1_2_7_21_1) 1969 e_1_2_7_42_1 e_1_2_7_12_1 e_1_2_7_44_1 e_1_2_7_10_1 Barsanti L. (e_1_2_7_4_1) 2006 e_1_2_7_48_1 e_1_2_7_27_1 South G. R. (e_1_2_7_46_1) 1987 e_1_2_7_29_1 Dubois M. (e_1_2_7_14_1) 1956; 18 e_1_2_7_51_1 e_1_2_7_30_1 e_1_2_7_53_1 e_1_2_7_32_1 e_1_2_7_22_1 e_1_2_7_34_1 e_1_2_7_20_1 e_1_2_7_36_1 e_1_2_7_38_1 Hansmann E. (e_1_2_7_24_1) 1973 |
| References_xml | – ident: e_1_2_7_15_1 doi: 10.1016/j.biortech.2011.04.006 – ident: e_1_2_7_34_1 doi: 10.1016/j.jphotobiol.2005.11.002 – ident: e_1_2_7_45_1 doi: 10.1038/265373a0 – ident: e_1_2_7_6_1 doi: 10.2134/jeq1974.00472425000300020003x – volume-title: Handbook of Phycological Methods, Culture Methods and Growth Measurements year: 1973 ident: e_1_2_7_24_1 contributor: fullname: Hansmann E. – ident: e_1_2_7_3_1 doi: 10.1016/j.biortech.2012.12.159 – ident: e_1_2_7_39_1 doi: 10.1016/0022-0981(72)90041-X – ident: e_1_2_7_33_1 doi: 10.1016/j.biombioe.2012.03.010 – ident: e_1_2_7_41_1 doi: 10.1111/j.0022-3646.1990.00393.x – ident: e_1_2_7_22_1 doi: 10.1111/j.1399-3054.1982.tb00352.x – ident: e_1_2_7_28_1 doi: 10.1111/j.1529-8817.2012.01154.x – ident: e_1_2_7_26_1 doi: 10.1016/0144-4565(86)90011-9 – ident: e_1_2_7_35_1 doi: 10.1016/S0044-8486(96)01416-0 – ident: e_1_2_7_7_1 doi: 10.4319/lo.1972.17.2.0265 – ident: e_1_2_7_18_1 doi: 10.1080/09670269300650071 – ident: e_1_2_7_40_1 doi: 10.1111/j.1399-3054.1971.tb01445.x – ident: e_1_2_7_52_1 doi: 10.1016/0044-8486(94)90125-2 – ident: e_1_2_7_53_1 doi: 10.1023/A:1012537219198 – ident: e_1_2_7_29_1 doi: 10.1111/j.1529-8817.2012.01177.x – ident: e_1_2_7_49_1 doi: 10.1080/09670262.2011.554886 – volume-title: Algae: Anatomy, Biochemistry and Biotechnology year: 2006 ident: e_1_2_7_4_1 contributor: fullname: Barsanti L. – volume: 18 start-page: 350 year: 1956 ident: e_1_2_7_14_1 article-title: Colorimetric method of determination of sugars and related substances publication-title: Analyt. Chem. doi: 10.1021/ac60111a017 contributor: fullname: Dubois M. – ident: e_1_2_7_42_1 doi: 10.1111/pre.12054 – ident: e_1_2_7_9_1 doi: 10.1046/j.1469-8137.1999.00539.x – ident: e_1_2_7_44_1 doi: 10.1016/0043-1354(76)90154-8 – volume-title: Methods for Chemical Analysis of Fresh Waters year: 1969 ident: e_1_2_7_21_1 contributor: fullname: Golterman H. L. – ident: e_1_2_7_51_1 doi: 10.4319/lo.1989.34.6.1014 – ident: e_1_2_7_19_1 doi: 10.2216/i0031-8884-36-5-345.1 – volume-title: Introduction to Phycology year: 1987 ident: e_1_2_7_46_1 contributor: fullname: South G. R. – ident: e_1_2_7_11_1 doi: 10.1007/s12010-008-8443-5 – ident: e_1_2_7_8_1 doi: 10.1080/00103627509366547 – ident: e_1_2_7_2_1 doi: 10.1016/0960-8524(91)90142-7 – ident: e_1_2_7_31_1 doi: 10.1111/j.0022-3646.1993.00587.x – ident: e_1_2_7_17_1 doi: 10.1007/BF02442457 – ident: e_1_2_7_47_1 doi: 10.1016/S0022-5320(69)90033-1 – ident: e_1_2_7_10_1 doi: 10.1023/A:1011925022941 – ident: e_1_2_7_12_1 doi: 10.1016/j.procbio.2005.03.006 – ident: e_1_2_7_25_1 doi: 10.1093/plankt/12.2.381 – volume: 28 start-page: 22 year: 1988 ident: e_1_2_7_50_1 article-title: Uptake and utilization of nitrogen compounds publication-title: Proc. Phytochem. Soc. Eur. contributor: fullname: Syrett P. J. – ident: e_1_2_7_32_1 doi: 10.1016/j.biortech.2010.06.036 – ident: e_1_2_7_5_1 doi: 10.1023/A:1007921728300 – ident: e_1_2_7_43_1 doi: 10.1016/j.apenergy.2012.03.027 – ident: e_1_2_7_27_1 doi: 10.1016/B0-12-176480-X/00353-3 – ident: e_1_2_7_36_1 doi: 10.2216/i0031-8884-41-2-158.1 – ident: e_1_2_7_38_1 doi: 10.1016/B978-0-12-372522-6.00007-4 – ident: e_1_2_7_37_1 doi: 10.1016/j.phytochem.2011.12.007 – ident: e_1_2_7_48_1 doi: 10.1016/0044-8486(91)90279-G – ident: e_1_2_7_23_1 doi: 10.1139/m62-029 – ident: e_1_2_7_13_1 doi: 10.1023/A:1026007902078 – ident: e_1_2_7_30_1 doi: 10.1007/BF01313275 – ident: e_1_2_7_20_1 doi: 10.1002/bit.260240308 – ident: e_1_2_7_16_1 doi: 10.1016/S0044-8486(98)00278-6 |
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| Title | Effect of nitrogenous resource on growth, biochemical composition and ultrastructure of I sochrysis galbana ( I sochrysidales, H aptophyta) |
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