Chimeric NP non coding regions between type A and C influenza viruses reveal their role in translation regulation

• Published in: PloS one Vol. 9; no. 9; p. e109046
• Main Authors: Crescenzo-Chaigne, Bernadette, Barbezange, Cyril, Frigard, Vianney, Poulain, Damien, van der Werf, Sylvie
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 30-09-2014; Public Library of Science (PLoS)
• Subjects: Animals; Base Sequence; Biochemistry, Molecular Biology; Biology and life sciences; Dogs; Gammainfluenzavirus - genetics; Gammainfluenzavirus - metabolism; Gene Expression Regulation, Viral; Genetics; Genomes; Genomics; Infectious diseases; Influenza; Influenza A virus - genetics; Influenza A virus - metabolism; Influenza viruses; Life Sciences; Madin Darby Canine Kidney Cells; Microbiology and Parasitology; Molecular biology; Molecular Sequence Data; mRNA; Mutagenesis; Mutagenesis, Site-Directed; Mutant Chimeric Proteins - chemistry; Mutant Chimeric Proteins - genetics; Mutant Chimeric Proteins - metabolism; Mutation; NP protein; Nucleocapsid Proteins; Orthomyxoviridae; Protein Biosynthesis; Reverse Genetics; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Viral - genetics; RNA, Viral - metabolism; RNA-Binding Proteins - chemistry; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Transcription, Genetic; Translation; Viral Core Proteins - chemistry; Viral Core Proteins - genetics; Viral Core Proteins - metabolism; Virology; Viruses; France
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0109046

Exchange of the non coding regions of the NP segment between type A and C influenza viruses was used to demonstrate the importance not only of the proximal panhandle, but also of the initial distal panhandle strength in type specificity. Both elements were found to be compulsory to rescue infectious virus by reverse genetics systems. Interestingly, in type A influenza virus infectious context, the length of the NP segment 5' NC region once transcribed into mRNA was found to impact its translation, and the level of produced NP protein consequently affected the level of viral genome replication.