The genotypic population structure of Mycobacterium tuberculosis complex from Moroccan patients reveals a predominance of Euro-American lineages
Tuberculosis (TB) remains a major health problem in Morocco. Characterization of circulating Mycobacterium tuberculosis genotypic lineages, important to understand the dynamic of the disease, was hereby addressed for the first time at a national level. Spoligotyping was performed on a panel of 592 M...
Saved in:
| Published in: | PloS one Vol. 7; no. 10; p. e47113 |
|---|---|
| Main Authors: | , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
Public Library of Science
15-10-2012
Public Library of Science (PLoS) |
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Tuberculosis (TB) remains a major health problem in Morocco. Characterization of circulating Mycobacterium tuberculosis genotypic lineages, important to understand the dynamic of the disease, was hereby addressed for the first time at a national level.
Spoligotyping was performed on a panel of 592 M. tuberculosis complex strains covering a 2-year period (2004-2006). It identified 129 patterns: 105 (n = 568 strains) corresponded to a SIT number in the SITVIT2 database, while 24 patterns were labeled as orphan. A total of 523 (88.3%) strains were clustered vs. 69 or 11.7% unclustered. Classification of strains within 3 large phylogenetical groups was as follows: group 1- ancestral/TbD1+/PGG1 (EAI, Bovis, Africanum), group 2- modern/TbD1-/PGG1 group (Beijing, CAS), group 3- evolutionary recent/TbD1-/PGG2/3 (Haarlem, X, S, T, LAM; alternatively designated as the Euro-American lineage). As opposed to group 3 strains (namely LAM, Haarlem, and T) that predominated (86.5% of all isolates), 6 strains belonged to group 2 (Beijing n = 5, CAS n = 1), and 3 strains (BOV_1 n = 2, BOV_4-CAPRAE) belonged to ancestral group 1 (EAI and AFRI lineage strains were absent). 12-loci MIRU-VNTR typing of the Casablanca subgroup (n = 114 strains) identified 71 patterns: 48 MITs and 23 orphan patterns; it allowed to reduce the clustering rate from 72.8% to 29.8% and the recent transmission rate from 64% to 20.2%.
The M. tuberculosis population structure in Morocco is highly homogeneous, and is characterized by the predominance of the Euro-American lineages, namely LAM, Haarlem, and T, which belong to the "evolutionary recent" TbD1-/PGG2/3 phylogenetic group. The combination of spoligotyping and MIRUs decreased the clustering rate significantly, and should now be systematically applied in larger studies. The methods used in this study appear well suited to monitor the M. tuberculosis population structure for an enhanced TB management program in Morocco. |
|---|---|
| AbstractList | Background Tuberculosis (TB) remains a major health problem in Morocco. Characterization of circulating Mycobacterium tuberculosis genotypic lineages, important to understand the dynamic of the disease, was hereby addressed for the first time at a national level. Methodology/Principal Findings Spoligotyping was performed on a panel of 592 M. tuberculosis complex strains covering a 2-year period (2004-2006). It identified 129 patterns: 105 (n = 568 strains) corresponded to a SIT number in the SITVIT2 database, while 24 patterns were labeled as orphan. A total of 523 (88.3%) strains were clustered vs. 69 or 11.7% unclustered. Classification of strains within 3 large phylogenetical groups was as follows: group 1- ancestral/TbD1+/PGG1 (EAI, Bovis, Africanum), group 2- modern/TbD1-/PGG1 group (Beijing, CAS), group 3- evolutionary recent/TbD1-/PGG2/3 (Haarlem, X, S, T, LAM; alternatively designated as the Euro-American lineage). As opposed to group 3 strains (namely LAM, Haarlem, and T) that predominated (86.5% of all isolates), 6 strains belonged to group 2 (Beijing n = 5, CAS n = 1), and 3 strains (BOV_1 n = 2, BOV_4-CAPRAE) belonged to ancestral group 1 (EAI and AFRI lineage strains were absent). 12-loci MIRU-VNTR typing of the Casablanca subgroup (n = 114 strains) identified 71 patterns: 48 MITs and 23 orphan patterns; it allowed to reduce the clustering rate from 72.8% to 29.8% and the recent transmission rate from 64% to 20.2%. Conclusion The M. tuberculosis population structure in Morocco is highly homogeneous, and is characterized by the predominance of the Euro-American lineages, namely LAM, Haarlem, and T, which belong to the "evolutionary recent" TbD1-/PGG2/3 phylogenetic group. The combination of spoligotyping and MIRUs decreased the clustering rate significantly, and should now be systematically applied in larger studies. The methods used in this study appear well suited to monitor the M. tuberculosis population structure for an enhanced TB management program in Morocco. Tuberculosis (TB) remains a major health problem in Morocco. Characterization of circulating Mycobacterium tuberculosis genotypic lineages, important to understand the dynamic of the disease, was hereby addressed for the first time at a national level. Spoligotyping was performed on a panel of 592 M. tuberculosis complex strains covering a 2-year period (2004-2006). It identified 129 patterns: 105 (n = 568 strains) corresponded to a SIT number in the SITVIT2 database, while 24 patterns were labeled as orphan. A total of 523 (88.3%) strains were clustered vs. 69 or 11.7% unclustered. Classification of strains within 3 large phylogenetical groups was as follows: group 1- ancestral/TbD1+/PGG1 (EAI, Bovis, Africanum), group 2- modern/TbD1-/PGG1 group (Beijing, CAS), group 3- evolutionary recent/TbD1-/PGG2/3 (Haarlem, X, S, T, LAM; alternatively designated as the Euro-American lineage). As opposed to group 3 strains (namely LAM, Haarlem, and T) that predominated (86.5% of all isolates), 6 strains belonged to group 2 (Beijing n = 5, CAS n = 1), and 3 strains (BOV_1 n = 2, BOV_4-CAPRAE) belonged to ancestral group 1 (EAI and AFRI lineage strains were absent). 12-loci MIRU-VNTR typing of the Casablanca subgroup (n = 114 strains) identified 71 patterns: 48 MITs and 23 orphan patterns; it allowed to reduce the clustering rate from 72.8% to 29.8% and the recent transmission rate from 64% to 20.2%. The M. tuberculosis population structure in Morocco is highly homogeneous, and is characterized by the predominance of the Euro-American lineages, namely LAM, Haarlem, and T, which belong to the "evolutionary recent" TbD1-/PGG2/3 phylogenetic group. The combination of spoligotyping and MIRUs decreased the clustering rate significantly, and should now be systematically applied in larger studies. The methods used in this study appear well suited to monitor the M. tuberculosis population structure for an enhanced TB management program in Morocco. BACKGROUNDTuberculosis (TB) remains a major health problem in Morocco. Characterization of circulating Mycobacterium tuberculosis genotypic lineages, important to understand the dynamic of the disease, was hereby addressed for the first time at a national level.METHODOLOGY/PRINCIPAL FINDINGSSpoligotyping was performed on a panel of 592 M. tuberculosis complex strains covering a 2-year period (2004-2006). It identified 129 patterns: 105 (n = 568 strains) corresponded to a SIT number in the SITVIT2 database, while 24 patterns were labeled as orphan. A total of 523 (88.3%) strains were clustered vs. 69 or 11.7% unclustered. Classification of strains within 3 large phylogenetical groups was as follows: group 1- ancestral/TbD1+/PGG1 (EAI, Bovis, Africanum), group 2- modern/TbD1-/PGG1 group (Beijing, CAS), group 3- evolutionary recent/TbD1-/PGG2/3 (Haarlem, X, S, T, LAM; alternatively designated as the Euro-American lineage). As opposed to group 3 strains (namely LAM, Haarlem, and T) that predominated (86.5% of all isolates), 6 strains belonged to group 2 (Beijing n = 5, CAS n = 1), and 3 strains (BOV_1 n = 2, BOV_4-CAPRAE) belonged to ancestral group 1 (EAI and AFRI lineage strains were absent). 12-loci MIRU-VNTR typing of the Casablanca subgroup (n = 114 strains) identified 71 patterns: 48 MITs and 23 orphan patterns; it allowed to reduce the clustering rate from 72.8% to 29.8% and the recent transmission rate from 64% to 20.2%.CONCLUSIONThe M. tuberculosis population structure in Morocco is highly homogeneous, and is characterized by the predominance of the Euro-American lineages, namely LAM, Haarlem, and T, which belong to the "evolutionary recent" TbD1-/PGG2/3 phylogenetic group. The combination of spoligotyping and MIRUs decreased the clustering rate significantly, and should now be systematically applied in larger studies. The methods used in this study appear well suited to monitor the M. tuberculosis population structure for an enhanced TB management program in Morocco. Tuberculosis (TB) remains a major health problem in Morocco. Characterization of circulating Mycobacterium tuberculosis genotypic lineages, important to understand the dynamic of the disease, was hereby addressed for the first time at a national level. Spoligotyping was performed on a panel of 592 M. tuberculosis complex strains covering a 2-year period (2004-2006). It identified 129 patterns: 105 (n = 568 strains) corresponded to a SIT number in the SITVIT2 database, while 24 patterns were labeled as orphan. A total of 523 (88.3%) strains were clustered vs. 69 or 11.7% unclustered. Classification of strains within 3 large phylogenetical groups was as follows: group 1- ancestral/TbD1+/PGG1 (EAI, Bovis, Africanum), group 2- modern/TbD1-/PGG1 group (Beijing, CAS), group 3- evolutionary recent/TbD1-/PGG2/3 (Haarlem, X, S, T, LAM; alternatively designated as the Euro-American lineage). As opposed to group 3 strains (namely LAM, Haarlem, and T) that predominated (86.5% of all isolates), 6 strains belonged to group 2 (Beijing n = 5, CAS n = 1), and 3 strains (BOV_1 n = 2, BOV_4-CAPRAE) belonged to ancestral group 1 (EAI and AFRI lineage strains were absent). 12-loci MIRU-VNTR typing of the Casablanca subgroup (n = 114 strains) identified 71 patterns: 48 MITs and 23 orphan patterns; it allowed to reduce the clustering rate from 72.8% to 29.8% and the recent transmission rate from 64% to 20.2%. The M. tuberculosis population structure in Morocco is highly homogeneous, and is characterized by the predominance of the Euro-American lineages, namely LAM, Haarlem, and T, which belong to the "evolutionary recent" TbD1-/PGG2/3 phylogenetic group. The combination of spoligotyping and MIRUs decreased the clustering rate significantly, and should now be systematically applied in larger studies. The methods used in this study appear well suited to monitor the M. tuberculosis population structure for an enhanced TB management program in Morocco. Background Tuberculosis (TB) remains a major health problem in Morocco. Characterization of circulating Mycobacterium tuberculosis genotypic lineages, important to understand the dynamic of the disease, was hereby addressed for the first time at a national level. Methodology/Principal Findings Spoligotyping was performed on a panel of 592 M. tuberculosis complex strains covering a 2-year period (2004–2006). It identified 129 patterns: 105 (n = 568 strains) corresponded to a SIT number in the SITVIT2 database, while 24 patterns were labeled as orphan. A total of 523 (88.3%) strains were clustered vs. 69 or 11.7% unclustered. Classification of strains within 3 large phylogenetical groups was as follows: group 1– ancestral/TbD1+/PGG1 (EAI, Bovis, Africanum), group 2– modern/TbD1−/PGG1 group (Beijing, CAS), group 3– evolutionary recent/TbD1−/PGG2/3 (Haarlem, X, S, T, LAM; alternatively designated as the Euro-American lineage). As opposed to group 3 strains (namely LAM, Haarlem, and T) that predominated (86.5% of all isolates), 6 strains belonged to group 2 (Beijing n = 5, CAS n = 1), and 3 strains (BOV_1 n = 2, BOV_4-CAPRAE) belonged to ancestral group 1 (EAI and AFRI lineage strains were absent). 12-loci MIRU-VNTR typing of the Casablanca subgroup (n = 114 strains) identified 71 patterns: 48 MITs and 23 orphan patterns; it allowed to reduce the clustering rate from 72.8% to 29.8% and the recent transmission rate from 64% to 20.2%. Conclusion The M. tuberculosis population structure in Morocco is highly homogeneous, and is characterized by the predominance of the Euro-American lineages, namely LAM, Haarlem, and T, which belong to the “evolutionary recent” TbD1−/PGG2/3 phylogenetic group. The combination of spoligotyping and MIRUs decreased the clustering rate significantly, and should now be systematically applied in larger studies. The methods used in this study appear well suited to monitor the M. tuberculosis population structure for an enhanced TB management program in Morocco. Background Tuberculosis (TB) remains a major health problem in Morocco. Characterization of circulating Mycobacterium tuberculosis genotypic lineages, important to understand the dynamic of the disease, was hereby addressed for the first time at a national level. Methodology/Principal Findings Spoligotyping was performed on a panel of 592 M. tuberculosis complex strains covering a 2-year period (2004–2006). It identified 129 patterns: 105 (n = 568 strains) corresponded to a SIT number in the SITVIT2 database, while 24 patterns were labeled as orphan. A total of 523 (88.3%) strains were clustered vs. 69 or 11.7% unclustered. Classification of strains within 3 large phylogenetical groups was as follows: group 1– ancestral/TbD1+/PGG1 (EAI, Bovis, Africanum), group 2– modern/TbD1−/PGG1 group (Beijing, CAS), group 3– evolutionary recent/TbD1−/PGG2/3 (Haarlem, X, S, T, LAM; alternatively designated as the Euro-American lineage). As opposed to group 3 strains (namely LAM, Haarlem, and T) that predominated (86.5% of all isolates), 6 strains belonged to group 2 (Beijing n = 5, CAS n = 1), and 3 strains (BOV_1 n = 2, BOV_4-CAPRAE) belonged to ancestral group 1 (EAI and AFRI lineage strains were absent). 12-loci MIRU-VNTR typing of the Casablanca subgroup (n = 114 strains) identified 71 patterns: 48 MITs and 23 orphan patterns; it allowed to reduce the clustering rate from 72.8% to 29.8% and the recent transmission rate from 64% to 20.2%. Conclusion The M. tuberculosis population structure in Morocco is highly homogeneous, and is characterized by the predominance of the Euro-American lineages, namely LAM, Haarlem, and T, which belong to the “evolutionary recent” TbD1−/PGG2/3 phylogenetic group. The combination of spoligotyping and MIRUs decreased the clustering rate significantly, and should now be systematically applied in larger studies. The methods used in this study appear well suited to monitor the M. tuberculosis population structure for an enhanced TB management program in Morocco. |
| Audience | Academic |
| Author | Filali-Maltouf, Abdelkarim Millet, Julie Chaoui, Imane Akrim, Mohammed El Aouad, Rajae Lahlou, Ouafae Rastogi, Nalin Sabouni, Radia El Mzibri, Mohammed |
| AuthorAffiliation | 3 Biology and Medical Research Unit, National Centre of Energy, Nuclear Sciences and Techniques, Rabat, Morocco St. Petersburg Pasteur Institute, Russian Federation 2 WHO Supranational TB Reference Laboratory, Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, Abymes, Guadeloupe, France 4 Laboratory of Microbiology and Molecular Biology, Faculty of Sciences, University of Mohammed V-Agdal, Rabat, Morocco 1 National Tuberculosis Reference Laboratory, National Institute of Hygiene, Rabat, Morocco |
| AuthorAffiliation_xml | – name: 1 National Tuberculosis Reference Laboratory, National Institute of Hygiene, Rabat, Morocco – name: 4 Laboratory of Microbiology and Molecular Biology, Faculty of Sciences, University of Mohammed V-Agdal, Rabat, Morocco – name: 3 Biology and Medical Research Unit, National Centre of Energy, Nuclear Sciences and Techniques, Rabat, Morocco – name: 2 WHO Supranational TB Reference Laboratory, Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, Abymes, Guadeloupe, France – name: St. Petersburg Pasteur Institute, Russian Federation |
| Author_xml | – sequence: 1 givenname: Ouafae surname: Lahlou fullname: Lahlou, Ouafae organization: National Tuberculosis Reference Laboratory, National Institute of Hygiene, Rabat, Morocco – sequence: 2 givenname: Julie surname: Millet fullname: Millet, Julie – sequence: 3 givenname: Imane surname: Chaoui fullname: Chaoui, Imane – sequence: 4 givenname: Radia surname: Sabouni fullname: Sabouni, Radia – sequence: 5 givenname: Abdelkarim surname: Filali-Maltouf fullname: Filali-Maltouf, Abdelkarim – sequence: 6 givenname: Mohammed surname: Akrim fullname: Akrim, Mohammed – sequence: 7 givenname: Mohammed surname: El Mzibri fullname: El Mzibri, Mohammed – sequence: 8 givenname: Nalin surname: Rastogi fullname: Rastogi, Nalin – sequence: 9 givenname: Rajae surname: El Aouad fullname: El Aouad, Rajae |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/23077552$$D View this record in MEDLINE/PubMed https://riip.hal.science/pasteur-00765837$$DView record in HAL |
| BookMark | eNqNk91q3DAQhU1JaX7aNyitoVDai91KK1mybwpLSJtAQqBNeytkebyrYFuufpbkLfrInc1uQjbkovhCZvydI-l45jDbG9wAWfaWkillkn65dskPupuOWJ4SwiWl7EV2QCs2m4gZYXuP3vezwxCuCSlYKcSrbH_GiJRFMTvI_l4tIV_A4OLtaE0-ujF1Olo35CH6ZGLykLs2v7g1rtYmgrepz2OqwZvUuWBDblw_dnCTt971-YXzzhg95COawBBD7mEFugu5zkcPjevtoAdz53mSvJvMe7RcCzo7gF5AeJ29bJGHN9v1KPv17eTq-HRyfvn97Hh-PjGSkTiptRA1bWkh8FjcyLJtG1M2XDDZykoSQyswhgGvGl6yRui6FYJzwJVSFLOj7P3Gd8RrqG2YQVE2E4UgGBMSZxuicfpajd722t8qp626Kzi_UNpHazpQjNdN3UBjeFVzVnOtKasYKSSjBRBSotfX7W6p7pHDaLzudkx3vwx2qRZuhc6SVoKjwWRjsHwiO52fq1GHCMkrQqQoSiZXFPlP2w29-5MgRNXbYKDr9AAu4T2xWypZVbxC9MMT9Pk0ttRC44Xt0Do8p1mbqjmvyllRVlIiNX2GwqeB3hrs1NZifUfweUeATISbuNApBHX288f_s5e_d9mPj9gltmBcBteldWuHXZBvQONdCB7ah3QpUetBu09DrQdNbQcNZe8e_9AH0f1ksX8CeCjt |
| CitedBy_id | crossref_primary_10_1128_genomeA_01756_16 crossref_primary_10_1371_journal_pone_0135695 crossref_primary_10_2807_1560_7917_ES_2019_24_4_1800005 crossref_primary_10_1007_s10344_016_1022_0 crossref_primary_10_1586_14787210_2014_974560 crossref_primary_10_1016_j_meegid_2017_08_003 crossref_primary_10_1371_journal_pone_0216306 crossref_primary_10_1016_j_idcr_2016_02_010 crossref_primary_10_1371_journal_pone_0253826 crossref_primary_10_1016_j_ijmyco_2013_06_003 crossref_primary_10_1186_s12879_018_3626_3 crossref_primary_10_1590_s0100_736x2017001100003 crossref_primary_10_1016_j_jgar_2017_07_002 crossref_primary_10_1590_s1678_9946202163019 crossref_primary_10_1155_2015_174732 crossref_primary_10_1186_s12879_021_06944_5 crossref_primary_10_1016_j_meegid_2013_05_023 crossref_primary_10_1016_j_rvsc_2014_02_009 crossref_primary_10_1089_mdr_2017_0160 crossref_primary_10_1371_journal_pone_0091023 crossref_primary_10_1016_j_tube_2015_11_002 |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2012 Public Library of Science Lahlou et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Distributed under a Creative Commons Attribution 4.0 International License 2012 Lahlou et al 2012 Lahlou et al |
| Copyright_xml | – notice: COPYRIGHT 2012 Public Library of Science – notice: Lahlou et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: Distributed under a Creative Commons Attribution 4.0 International License – notice: 2012 Lahlou et al 2012 Lahlou et al |
| DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION IOV ISR 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PIMPY PQEST PQQKQ PQUKI PTHSS PYCSY RC3 7X8 1XC VOOES 5PM DOA |
| DOI | 10.1371/journal.pone.0047113 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Opposing Viewpoints (Gale) Gale In Context: Science ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest Central Advanced Technologies & Aerospace Collection Agricultural & Environmental Science Collection ProQuest Central Essentials Biological Science Collection ProQuest Central Technology Collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Materials Science Collection ProQuest Central Korea Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection Biological Sciences Agriculture Science Database Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Materials Science Collection Publicly Available Content Database ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition Engineering Collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic Hyper Article en Ligne (HAL) Hyper Article en Ligne (HAL) (Open Access) PubMed Central (Full Participant titles) Directory of Open Access Journals |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection Environmental Sciences and Pollution Management Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Biological Science Collection ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic Technology Collection Technology Research Database Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE Agricultural Science Database |
| Database_xml | – sequence: 1 dbid: DOA name: Directory of Open Access Journals url: http://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: ECM name: MEDLINE url: https://search.ebscohost.com/login.aspx?direct=true&db=cmedm&site=ehost-live sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Sciences (General) Public Health Biology Medicine |
| DocumentTitleAlternate | M. tuberculosis Population Structure in Morocco |
| EISSN | 1932-6203 |
| Editor | Mokrousov, Igor |
| Editor_xml | – sequence: 1 givenname: Igor surname: Mokrousov fullname: Mokrousov, Igor |
| EndPage | e47113 |
| ExternalDocumentID | 1326560866 oai_doaj_org_article_34bdbdedc49b43b4aa1393057315e008 oai_HAL_pasteur_00765837v1 2943730121 A498258977 10_1371_journal_pone_0047113 23077552 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GeographicLocations | Morocco Guadeloupe |
| GeographicLocations_xml | – name: Morocco – name: Guadeloupe |
| GroupedDBID | --- 123 29O 2WC 3V. 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ ABDBF ABIVO ABJCF ABUWG ACGFO ACIHN ACIWK ACPRK ADBBV ADRAZ AEAQA AENEX AFKRA AFRAH AHMBA ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAWUL BBNVY BBORY BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CGR CS3 CUY CVF D1I D1J D1K DIK DU5 E3Z EAP EAS EBD ECM EIF EMOBN ESTFP ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IHR IHW INH INR IOV IPNFZ IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ NPM O5R O5S OK1 P2P P62 PATMY PDBOC PIMPY PQQKQ PROAC PSQYO PTHSS PV9 PYCSY RIG RNS RPM RZL SV3 TR2 UKHRP WOQ WOW ~02 ~KM AAYXX CITATION AFPKN 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K DWQXO FR3 GNUQQ H94 K9. KL. M7N P64 PQEST PQUKI RC3 7X8 1XC VOOES 5PM AAPBV ABPTK BBAFP N95 |
| ID | FETCH-LOGICAL-c730t-ba66b1f156bac4c78ffdc8d4637f7970c19ecc3e49d483d6abf6644eabf11ba63 |
| IEDL.DBID | RPM |
| ISSN | 1932-6203 |
| IngestDate | Sun Nov 06 00:11:22 EDT 2022 Tue Oct 22 15:16:47 EDT 2024 Tue Sep 17 20:56:18 EDT 2024 Tue Oct 15 15:59:05 EDT 2024 Fri Oct 25 22:04:44 EDT 2024 Sat Nov 09 15:06:49 EST 2024 Tue Nov 19 20:57:19 EST 2024 Tue Nov 12 22:47:46 EST 2024 Thu Aug 01 20:36:46 EDT 2024 Thu Aug 01 20:05:33 EDT 2024 Tue Aug 20 22:14:06 EDT 2024 Thu Nov 21 21:27:14 EST 2024 Sat Nov 02 12:21:40 EDT 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 10 |
| Language | English |
| License | Distributed under a Creative Commons Attribution 4.0 International License: http://creativecommons.org/licenses/by/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. Creative Commons Attribution License |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c730t-ba66b1f156bac4c78ffdc8d4637f7970c19ecc3e49d483d6abf6644eabf11ba63 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Conceived and designed the experiments: OL IC NR REA. Performed the experiments: OL JM IC RS MA. Analyzed the data: OL JM IC RS MA NR REA. Contributed reagents/materials/analysis tools: OL IC AFM MEM NR REA. Wrote the paper: OL JM NR REA. Data entry and comparison using the SITVIT2 proprietary database of Institut Pasteur de la Guadeloupe: NR. Competing Interests: The authors have declared that no competing interests exist. |
| ORCID | 0000-0002-7199-7747 |
| OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471964/ |
| PMID | 23077552 |
| PQID | 1326560866 |
| PQPubID | 1436336 |
| PageCount | e47113 |
| ParticipantIDs | plos_journals_1326560866 doaj_primary_oai_doaj_org_article_34bdbdedc49b43b4aa1393057315e008 pubmedcentral_primary_oai_pubmedcentral_nih_gov_3471964 hal_primary_oai_HAL_pasteur_00765837v1 proquest_miscellaneous_1113979949 proquest_journals_1326560866 gale_infotracmisc_A498258977 gale_infotracacademiconefile_A498258977 gale_incontextgauss_ISR_A498258977 gale_incontextgauss_IOV_A498258977 gale_healthsolutions_A498258977 crossref_primary_10_1371_journal_pone_0047113 pubmed_primary_23077552 |
| PublicationCentury | 2000 |
| PublicationDate | 2012-10-15 |
| PublicationDateYYYYMMDD | 2012-10-15 |
| PublicationDate_xml | – month: 10 year: 2012 text: 2012-10-15 day: 15 |
| PublicationDecade | 2010 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: San Francisco – name: San Francisco, USA |
| PublicationTitle | PloS one |
| PublicationTitleAlternate | PLoS One |
| PublicationYear | 2012 |
| Publisher | Public Library of Science Public Library of Science (PLoS) |
| Publisher_xml | – name: Public Library of Science – name: Public Library of Science (PLoS) |
| SSID | ssj0053866 |
| Score | 2.2434678 |
| Snippet | Tuberculosis (TB) remains a major health problem in Morocco. Characterization of circulating Mycobacterium tuberculosis genotypic lineages, important to... Background Tuberculosis (TB) remains a major health problem in Morocco. Characterization of circulating Mycobacterium tuberculosis genotypic lineages,... BACKGROUNDTuberculosis (TB) remains a major health problem in Morocco. Characterization of circulating Mycobacterium tuberculosis genotypic lineages, important... BACKGROUND: Tuberculosis (TB) remains a major health problem in Morocco. Characterization of circulating Mycobacterium tuberculosis genotypic lineages,... Background Tuberculosis (TB) remains a major health problem in Morocco. Characterization of circulating Mycobacterium tuberculosis genotypic lineages,... |
| SourceID | plos doaj pubmedcentral hal proquest gale crossref pubmed |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database |
| StartPage | e47113 |
| SubjectTerms | Adolescent Adult Age Aged Aged, 80 and over Analysis Bacterial genetics Bacteriology Biology Child Cloning Cluster Analysis Clustering Deoxyribonucleic acid Disease transmission DNA DNA, Bacterial - genetics Drug resistance Epidemiology Genetic aspects Genotype Genotype & phenotype Health surveillance Humans Hygiene Infections Laboratories Life Sciences Males Medical research Medicine Methods Microbiology and Parasitology Middle Aged Morocco - epidemiology Mycobacterium tuberculosis Mycobacterium tuberculosis - classification Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Phylogeny Population Population structure Public health Spoligotyping Strains (organisms) Studies Supranationalism Tuberculosis Tuberculosis - blood Tuberculosis - epidemiology Tuberculosis - microbiology Young Adult |
| SummonAdditionalLinks | – databaseName: Directory of Open Access Journals dbid: DOA link: http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELbonrggyquBAgYhHoe0Tew4yXGBVotEQaKAuFl-sitBEm02iP4LfjIzTjZqUCU4cFppPbux5-WZeOYzIU-USnlmlYqZSU0MO7SOS1sISFWU9Vwb4wLO7OIsf_eleH2MMDnjVV9YE9bDA_eMO2RcW22dNbzUnGmuFMQsDGH8ksxt23yPxDaZ6n0wWLEQQ6Mcy5PDQS4HTV25AwRITBI22YgCXv_olXeWWBQ5a77V7WWB55_1kxc2pJPr5NoQSdJ5v4JdcsVVN8juYKstfT4ASr-4SX6BLlAEY92cNytDm_HOLtqjx3ZrR2tPT88NGHcAb-6-002n3dp0MLlVS0PhuftJsRmFntaw6YFE6IDJ2lKEgQI1poo2iD8aqmtM-E982R9vT4UohrTgv9pb5NPJ8cdXi3i4iSE24AE2sVZC6MRDrgfT4CYvvLemsFyw3OdlfmSSElSBOV5aXjArlPYCAi0Hn0kCP2a3yawC3u8RKrzOU8Zt6YFcO18wr1wK6XBWCgPqEZF4KxbZ9IAbMpy65ZCo9JyVKEY5iDEiL1F2Iy3CZYcvQInkoETyb0oUkYcoedn3no5GL-e8hAy6gBg5Io8DBUJmVFiT81V1bSvfvP_8D0RnHyZEzwYiX4P2GDX0QcCaEIprQrk_oQTDN5Php0tkxoWFL-ZvZaPAPXRriWesWcHyH0lE9lCVt-xrgZkpYi6BjcAjtup9-fCjcRifjgV5lau7FhPHcCLMy4jc6a1hnAn2FuRZlkYkn9jJZKrTkWq1DNDmDGRaCn73fwj1HrkK0W2KgUaS7ZMZWJS7T3Za2z0IzuI33VxzmA priority: 102 providerName: Directory of Open Access Journals |
| Title | The genotypic population structure of Mycobacterium tuberculosis complex from Moroccan patients reveals a predominance of Euro-American lineages |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/23077552 https://www.proquest.com/docview/1326560866 https://search.proquest.com/docview/1113979949 https://riip.hal.science/pasteur-00765837 https://pubmed.ncbi.nlm.nih.gov/PMC3471964 https://doaj.org/article/34bdbdedc49b43b4aa1393057315e008 http://dx.doi.org/10.1371/journal.pone.0047113 |
| Volume | 7 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lj9MwELZoJRAXxJbHBpbFIMTjkJbEzutYll0ViUK1C4hbZDvOtlKbRE2zov-Cn8yM89AG7QFxqlpPmonn4ZnM-DMhr4RwuZcIYTPlKhtWaGlHSehDqiKSlEultMGZnV0EX36GH08RJsdr98KYpn0lV-NsvRlnq6XprSw2atL2iU0W8xMGHjXy-WRABhAbtil67X7BgH2_2SPHAmfSiGRc5JkeIzai4-DZOdj_HHie21uODGp_55sHS2yNHBbrvLwp_Py7i_LasnR2n9xr4kk6rfk-ILd0NiK36xMm9yNyZ97UzkfkoDHjkr5tsKbfPSC_QU0ofM13-2Kl6KI7zoteGGDZaqtpntL5XoHdG1znakN3ldRbVQHHq5KiQ1nrXxT3qdB5DushCIsuarjWkp7rKwhFSyroAqFJTeONMv-JdQC7LRhRyIk1uLbyIfl-dvrtZGY3hzTYCpzDzpbC96WTQhoIbHAVhGmaqDDhPgvSIAreKycCLWGaRwkPWeILmfoQg2n4dBy4mD0iwwxkc0ion8rAZTyJUiCXOg1ZKrQLmbIX-Qo0xyJ2K6u4qLE4YlOQCyCHqac7RjHHjZgt8gEF2tEikrb5Id9exo0-xYzLRCYgRR5JziQXAmJihjCRjqchQLLIc1SHuN6W2vmDeMojSK5DCJ8t8tJQIJpGhu06l6Iqy_jT1x__QHRx3iN60xClOaiUEs0WCXgmROnqUR71KMEnqN7w6yVOxrUHn00_x4UAz1FtYyy_eiELrhyLHKJ-t9NXwmS6CMcENgS3aHX-5uEX3TDeHXv1Mp1XJeaUpljMI4s8rk2k46Q1O4sEPePpsdofASdgUM8bo3_y31c-JXch2nUx8HC8IzIEM9LPyKBMqmPzJubY-JE_bUp6Qg |
| link.rule.ids | 230,315,729,782,786,866,887,2106,27933,27934,53800,53802 |
| linkProvider | National Library of Medicine |
| linkToHtml | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELZoEY8L0PJooFCDEI9Ddjex8zqW0morumXVFsQtsh2nXWk3iTabiv4LfjIzzkMN6gH1tEo82cT2zOcZzfgzIe-FcLmXCGEz5SobVmhpR0noQ6gikpRLpbThmR2fBse_wq_7SJPjtXthTNG-krNBNl8MstmFqa0sFmrY1okNp5M9Boga-Xy4Ru6CvY5GbZBeAzDc8v1mlxwLnGEzKYMiz_QA2REdB0_PwQrowPPc3oJkePs7dF67wOLI9WKelzc5oP_WUV5bmA4e37JLT8ijxhOlu3XzBrmjs01yrz6b8mqT3J80WfdNstEAQEk_NSzVn5-SP6BgFC7z1VUxU3TaHQRGTw0lbbXUNE_p5EoBYhhG6GpBV5XUS1VBT2clRSia698Ud7jQSQ4rKUwzndZEryU90ZfgxJZU0CmSmpqSHWX-EzMIdptqohBNawDF8hn5cbB_tje2m-MdbAWwsrKl8H3ppBBAwmdwFYRpmqgw4T4L0iAKRsqJQL-Y5lHCQ5b4QqY-eG8afh0HHmbPyXoGc7pFqJ_KwGU8iVIQlzoNWSq0CzG2F_kKdM4idjvHcVGzeMQmlRdA9FMPd4zqETfqYZEvqAidLHJwmxv58jxupi1mXCYygdnnkeRMciHAm2ZIMOl4Glwri-ygGsX1htYOSeJdHkFYHoLjbZF3RgJ5ODIs9DkXVVnGh99__ofQ6UlP6GMjlOagiko0myugT8jv1ZPc7kkCmqhe84cLHIxrHR_vHsWFAMypljEmbr2QBZeORbbQLtrhK2EwXSRyAtuDV7S2cnPz264Z345VfpnOqxKjUZNm5pFFXtSm1X1Ja64WCXpG1_vUfgvYmuFLb2zr5a2f3CEPxmeTo_jo8PjbK_IQfGYX3RfH2ybrYFL6NVkrk-qNQaG_x8KO1w |
| linkToPdf | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwELboIiouQMujgUINQjwO2W1i53UsfagV3bJqAXGLbMduV2qTaNNU9F_wk5lxHmpQDwhOq8STTWzPfJ7RjD8T8lYInweZEC5TvnJhhZZuksUhhCoiM1wqpS3P7P5JdPQj3tlFmpz-qC9btK_kfJyfX4zz-ZmtrSwv1KSrE5vMptsMEDUJ-aTMzGSJ3AWb3fS7QL0BYbgVhu1OORZ5k3ZixmWR6zEyJHoenqCDVdBREPiDRcly9_cIvXSGBZKj8ryobnNC_6ylvLE47T38j249Ig9aj5RuNSIr5I7OV8m95ozK61WyPG2z76tkpQWCin5o2ao_Pia_QNEoXBaX1-Vc0Vl_IBg9sdS09ULTwtDptQLksMzQ9QW9rKVeqBp6O68oQtK5_klxpwudFrCiwnTTWUP4WtFjfQXObEUFnSG5qS3dUfY_MZPgdiknClG1BnCsnpBve7tft_fd9pgHVwG8XLpShKH0DASS8BlcRbExmYozHrLIREm0qbwE9IxpnmQ8ZlkopAnBi9Pw63nwMHtKRjnM6xqhoZGRz3iWGBCX2sTMCO1DrB0koQLdc4jbzXNaNmweqU3pRRAFNcOdooqkrYo45BMqQy-LXNz2RrE4TdupSxmXmcxAA3giOZNcCPCqGRJNeoEGF8shG6hKabOxtUeUdIsnEJ7H4IA75I2VQD6OHAt-TkVdVenBl-9_IXRyPBB63wqZAtRRiXaTBfQJeb4GkusDSUAVNWh-d4aDcaPj-1uHaSkAe-pFigncIGbRleeQNbSNbvgqGEwfCZ3A_uAVnb3c3vy6b8a3Y7Vfrou6wqjUppt54pBnjXn1X9KZrEOigeENPnXYAvZmedNb-3r-z09ukOXZzl56eHD0-QW5D66zj16MF6yTEViUfkmWqqx-ZYHoNwGHkVc |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+Genotypic+Population+Structure+of+Mycobacterium+tuberculosis+Complex+from+Moroccan+Patients+Reveals+a+Predominance+of+Euro-American+Lineages&rft.jtitle=PloS+one&rft.au=Lahlou%2C+Ouafae&rft.au=Millet%2C+Julie&rft.au=Chaoui%2C+Imane&rft.au=Sabouni%2C+Radia&rft.date=2012-10-15&rft.pub=Public+Library+of+Science&rft.eissn=1932-6203&rft.volume=7&rft.issue=10&rft.spage=e47113&rft_id=info:doi/10.1371%2Fjournal.pone.0047113&rft.externalDBID=HAS_PDF_LINK&rft.externalDocID=2943730121 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon |