Fruit-specific RNAi-mediated suppression of DET1 enhances carotenoid and flavonoid content in tomatoes

• Published in: Nature biotechnology Vol. 23; no. 7; pp. 890 - 895
• Main Authors: Davuluri, G.R, Tuinen, A. van, Fraser, P.D, Manfredonia, A, Newman, R, Burgess, D, Brummell, D.A, King, S.R, Palys, J, Uhlig, J
• Format: Journal Article
• Language: English
• Published: United States Nature Publishing Group 01-07-2005
• Subjects: biosynthesis; Cardiovascular disease; Carotenoids; Carotenoids - biosynthesis; degradation; DET1 gene; DNA binding proteins; Down-Regulation; Enzymes; Flavonoids; Flavonoids - biosynthesis; Food; fruit-specific promoters; Fruits; Fruits (Food); Gene Expression Regulation, Plant; gene silencing; Genes; Genetic aspects; Genetic engineering; homeotic genes; Isoflavones; Lycopersicon esculentum; Lycopersicon esculentum - genetics; Lycopersicon esculentum - metabolism; messenger RNA; Metabolites; Novels; Nutrition; Nutritive Value; Observations; Physiological aspects; Phytochemicals; Plant genetics; Plant Proteins - antagonists & inhibitors; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; promoter regions; RNA; RNA Interference; Solanum lycopersicum var. lycopersicum; Technology; Tomatoes; Transcription (Genetics); transcription factors; Transgenic plants; Vegetables; Italy; United States > US; Texas; California
• ISSN: 1087-0156; 1546-1696
• DOI: 10.1038/nbt1108

Tomatoes are a principal dietary source of carotenoids and flavonoids, both of which are highly beneficial for human health. Overexpression of genes encoding biosynthetic enzymes or transcription factors have resulted in tomatoes with improved carotenoid or flavonoid content, but never with both. We attempted to increase tomato fruit nutritional value by suppressing an endogenous photomorphogenesis regulatory gene, DET1, using fruit-specific promoters combined with RNA interference (RNAi) technology. Molecular analysis indicated that DET1 transcripts were indeed specifically degraded in transgenic fruits. Both carotenoid and flavonoid contents were increased significantly, whereas other parameters of fruit quality were largely unchanged. These results demonstrate that manipulation of a plant regulatory gene can simultaneously influence the production of several phytonutrients generated from independent biosynthetic pathways, and provide a novel example of the use of organ-specific gene silencing to improve the nutritional value of plant-derived products.