Optimization of 1-deoxynojirimycin extraction from mulberry leaves by using response surface methodology

• Published in: Bioscience, biotechnology, and biochemistry Vol. 73; no. 12; pp. 2684 - 2689
• Main Authors: Vichasilp, C., Tohoku Univ., Sendai (Japan), Nakagawa, K, Sookwong, P, Suzuki, Y, Kimura, F, Higuchi, O, Miyazawa, T
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01-12-2009; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: 1-deoxynojirimycin (DNJ); 1-Deoxynojirimycin - analysis; 1-Deoxynojirimycin - chemistry; 1-Deoxynojirimycin - isolation & purification; Biological and medical sciences; Chemical Fractionation - methods; Chromatography; DIABETE; DIABETES; EXTRACCION; EXTRACTION; FEUILLE; Fundamental and applied biological sciences. Psychology; GLICOSIDASAS; GLUCOSA; GLUCOSE; GLYCOSIDASE; GLYCOSIDASES; HOJAS; http://www.fao.org/aos/agrovoc#c_10361; http://www.fao.org/aos/agrovoc#c_3287; http://www.fao.org/aos/agrovoc#c_3308; http://www.fao.org/aos/agrovoc#c_36910; http://www.fao.org/aos/agrovoc#c_4243; http://www.fao.org/aos/agrovoc#c_4949; http://www.fao.org/aos/agrovoc#c_8044; LEAVES; Morus - chemistry; MORUS ALBA; mulberry; Plant Leaves - chemistry; response surface methodology (RSM); Tandem Mass Spectrometry; ULTRASON; ULTRASONICS; ULTRASONIDO; ultrasound-assisted extraction (UAE); Statistical method; 1-deoxynojirimycin (DNJ); Response surface; Extraction; Plant leaf; mulberry; Ultrasound; Optimization; ultrasound-assisted extraction (UAE); response surface methodology (RSM)
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.90543

Mulberry 1-deoxynojirimycin (DNJ, a potent α-glycosidase inhibitor) has therapeutic potency against diabetes mellitus. However, the amount of DNJ in mulberry leaves is low (about 0.1%), and a more effective extraction method is needed. Ultrasound-assisted extraction (UAE) was applied in this study for mulberry DNJ extraction, and five factors, the percentage of ethanol in the extraction solvent (x
1
), ratio of the extraction solvent to mulberry sample (x
2
), ultrasonic power (x
3
), extraction temperature (x
4
) and extraction time (x
5
), were investigated by fractional factorial 2
(5-1)
design (FFD) to obtain the optimum extraction efficiency (DNJ yield, Y
1
) and extraction productivity (total yield, Y
2
). The results showed that x
2
, x
3
and x
5
had significant impact on Y
1
and Y
2
, and were further optimized by response surface methodology (RSM). Under the optimized conditions (x
2
, x
3
and x
5
of 7 ml/g, 180 W and 260 s, respectively), DNJ-enriched powder (0.8%) was produced with high extraction efficiency (98%) and productivity (20%), enabling this product to be used for nutraceutical purposes.