Development of Radioimmunoassay for Measurement of Serum Digoxin in Digitalized Patients Using Novel Anti-digoxin Antiserum

There is an antiserum elicited by digoxin 3′-hemisuccinate–bovine serum albumin (BSA) conjugate possessing high specificity for digoxin. Our study focused on development of RIA using this novel antiserum for measurement of digoxin in serum from digitalized patients. The property of the new antiserum...

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Published in:	Biological & pharmaceutical bulletin Vol. 25; no. 4; pp. 422 - 425
Main Authors:	Ikeda, Yukari, Araki, Tsutomu, Takimoto, Hiroaki, Fujii, Youichi
Format:	Journal Article
Language:	English
Published:	Tokyo The Pharmaceutical Society of Japan 01-04-2002 Maruzen Japan Science and Technology Agency
Subjects:	Analysis Animals anti-digoxin antiserum Biological and medical sciences Cardiotonic agents Cardiovascular system Cattle digoxin Digoxin - analogs & derivatives Digoxin - analysis Digoxin - blood digoxin 3′-hemisuccinate–bovine serum albumin (BSA) conjugate General pharmacology Humans Immune Sera - analysis Immune Sera - blood Medical sciences Pharmacology. Drug treatments Radioimmunoassay - methods Radioimmunoassay - statistics & numerical data RIA Serum Albumin, Bovine - analysis Human Biological fluid Cardiotonic agent Digoxin Free form Monoclonal antibody Blood Bound form Absorption Antiserum Analysis method Reproducibility Radioimmunoassay Distribution Separation process Serum Glycoside Pharmacokinetics Digitalis drug Quantitative analysis
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Abstract	There is an antiserum elicited by digoxin 3′-hemisuccinate–bovine serum albumin (BSA) conjugate possessing high specificity for digoxin. Our study focused on development of RIA using this novel antiserum for measurement of digoxin in serum from digitalized patients. The property of the new antiserum was investigated by RIA with digoxin 3′-hemisuccinyl-[3H]leucine. The separation of bound and free fractions was performed using a dextran-coated charcoal suspension. The new antiserum bound approximately 50% of digoxin 3′-hemisuccinyl-[3H]leucine with a final dilution of 1 : 30000. The intra- and inter-assay coefficients of variation were <9% in the range of 0.52—4.17 ng/ml. The mean digoxin concentration in serum samples (n=35) from digitalized patients was estimated to be 0.68 ng/ml, which was lower than its measurement of digoxin with the commercial anti-digoxin BSA serum and monoclonal anti-digoxin. It is apparent that the RIA described here has sufficient precision. The RIA system was available for the measurement of digoxin in serum from digitalized patients.
AbstractList	There is an antiserum elicited by digoxin 3′-hemisuccinate–bovine serum albumin (BSA) conjugate possessing high specificity for digoxin. Our study focused on development of RIA using this novel antiserum for measurement of digoxin in serum from digitalized patients. The property of the new antiserum was investigated by RIA with digoxin 3′-hemisuccinyl-[3H]leucine. The separation of bound and free fractions was performed using a dextran-coated charcoal suspension. The new antiserum bound approximately 50% of digoxin 3′-hemisuccinyl-[3H]leucine with a final dilution of 1 : 30000. The intra- and inter-assay coefficients of variation were <9% in the range of 0.52—4.17 ng/ml. The mean digoxin concentration in serum samples (n=35) from digitalized patients was estimated to be 0.68 ng/ml, which was lower than its measurement of digoxin with the commercial anti-digoxin BSA serum and monoclonal anti-digoxin. It is apparent that the RIA described here has sufficient precision. The RIA system was available for the measurement of digoxin in serum from digitalized patients. There is an antiserum elicited by digoxin 3'-hemisuccinate-bovine serum albumin (BSA) conjugate possessing high specificity for digoxin. Our study focused on development of RIA using this novel antiserum for measurement of digoxin in serum from digitalized patients. The property of the new antiserum was investigated by RIA with digoxin 3'-hemisuccinyl-[3H]leucine. The separation of bound and free fractions was performed using a dextran-coated charcoal suspension. The new antiserum bound approximately 50% of digoxin 3'-hemisuccinyl-[3H]leucine with a final dilution of 1:30000. The intra- and inter-assay coefficients of variation were <9% in the range of 0.52-4.17 ng/ml. The mean digoxin concentration in serum samples (n=35) from digitalized patients was estimated to be 0.68 ng/ml, which was lower than its measurement of digoxin with the commercial antidigoxin BSA serum and monoclonal anti-digoxin. It is apparent that the RIA described here has sufficient precision. The RIA system was available for the measurement of digoxin in serum from digitalized patients. There is an antiserum elicited by digoxin 3'-hemisuccinate-bovine serum albumin (BSA) conjugate possessing high specificity for digoxin. Our study focused on development of RIA using this novel antiserum for measurement of digoxin in serum from digitalized patients. The property of the new antiserum was investigated by RIA with digoxin 3'-hemisuccinyl-[3H]leucine. The separation of bound and free fractions was performed using a dextran-coated charcoal suspension. The new antiserum bound approximately 50% of digoxin 3'-hemisuccinyl-[3H]leucine with a final dilution of 1:30000. The intra- and inter-assay coefficients of variation were <9% in the range of 0.52-4.17 ng/ml. The mean digoxin concentration in serum samples (n=35) from digitalized patients was estimated to be 0.68 ng/ml, which was lower than its measurement of digoxin with the commercial antidigoxin BSA serum and monoclonal anti-digoxin. It is apparent that the RIA described here has sufficient precision. The RIA system was available for the measurement of digoxin in serum from digitalized patients.
Author	Araki, Tsutomu Fujii, Youichi Takimoto, Hiroaki Ikeda, Yukari
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Cites_doi	10.1093/ajcp/82.2.210 10.1093/clinchem/32.1.5 10.1111/j.1749-6632.1949.tb27297.x 10.1093/clinchem/34.2.393 10.1093/clinchem/40.1.172 10.1016/0009-8981(77)90228-5 10.1007/BF01177965 10.1089/hyb.1990.9.619 10.1210/jcem-29-6-866 10.1097/00007691-199612000-00008 10.1093/clinchem/40.7.1348 10.1248/bpb.23.906 10.1111/j.2042-7158.1977.tb11232.x 10.1073/pnas.57.1.71 10.1021/bi00804a020 10.1038/clpt.1984.11 10.1093/clinchem/40.10.1898
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References_xml	– volume: 82 start-page: 210 issn: 0002-9173 year: 1984 ident: 6 publication-title: Am. J. Clin. Pathol. doi: 10.1093/ajcp/82.2.210 contributor: fullname: Valdes R., Jr., Brown B. A., Graves S. W. – volume: 32 start-page: 5 year: 1986 ident: 7 publication-title: Clin. Chem. doi: 10.1093/clinchem/32.1.5 contributor: fullname: Soldin S. J. – volume: 51 start-page: 660 issn: 0077-8923 year: 1949 ident: 15 publication-title: Ann. N.Y. Acad. Sci. doi: 10.1111/j.1749-6632.1949.tb27297.x contributor: fullname: Scatchard G. – volume: 34 start-page: 393 year: 1988 ident: 17 publication-title: Clin. Chem. doi: 10.1093/clinchem/34.2.393 contributor: fullname: Bednarczyk B., Soldin S. J., Gasinska I., D'Costa M., Perrot L. – volume: 40 start-page: 172 year: 1994 ident: 11 publication-title: Clin. Chem. doi: 10.1093/clinchem/40.1.172 contributor: fullname: Fujii Y., Ikeda Y., Yamazaki M. – ident: 9 doi: 10.1016/0009-8981(77)90228-5 – volume: 184 start-page: 424 issn: 0022-3565 year: 1973 ident: 3 publication-title: J. Pharmacol. Exp. Ther. contributor: fullname: Watson E., Clark D. R., Kalman S. M. – volume: 44 start-page: 633 issn: 0028-1042 year: 1957 ident: 13 publication-title: Naturwissenschaften doi: 10.1007/BF01177965 contributor: fullname: Haack E., Kaiser F., Spingler H. – volume: 9 start-page: 619 issn: 0272-457X issue: 6 year: 1990 ident: 16 publication-title: Hybridoma doi: 10.1089/hyb.1990.9.619 – volume: 29 start-page: 866 issn: 0021-972X year: 1969 ident: 14 publication-title: J. Clin. Endocrinol. Metab. doi: 10.1210/jcem-29-6-866 contributor: fullname: Abraham G. E. – ident: 10 doi: 10.1097/00007691-199612000-00008 – volume: 40 start-page: 1348 year: 1994 ident: 18 publication-title: Clin. Chem. doi: 10.1093/clinchem/40.7.1348 contributor: fullname: Datta P., Larsen F. – volume: 23 start-page: 906 issn: 0918-6158 issue: 8 year: 2000 ident: 12 publication-title: Biol. Pharm. Bull. doi: 10.1248/bpb.23.906 contributor: fullname: IKEDA Y – volume: 29 start-page: 27 issn: 0022-3573 issue: 1 year: 1977 ident: 1 publication-title: J. Pharm. Pharmacol. doi: 10.1111/j.2042-7158.1977.tb11232.x contributor: fullname: Gault M. H., Charles J. D., Sugden D. L., Kepkay D. C. – volume: 57 start-page: 71 issn: 0027-8424 year: 1967 ident: 4 publication-title: Proc. Natl. Acad. Sci. U.S.A. doi: 10.1073/pnas.57.1.71 contributor: fullname: Butler V. P., Jr., Chen J. P. – volume: 9 start-page: 331 issn: 0006-2960 year: 1970 ident: 5 publication-title: Biochemistry doi: 10.1021/bi00804a020 contributor: fullname: Smith T. W., Butler V. P., Jr., Haber E. – volume: 35 start-page: 74 issn: 0009-9236 issue: 1 year: 1984 ident: 2 publication-title: Clin. Pharmacol. Ther. doi: 10.1038/clpt.1984.11 contributor: fullname: Gault M. H., Longerich L. L., Loo J. C. K., Ko P. T. H., Fine A., Vasdev S. C., Dawe M. A. – volume: 40 start-page: 1898 year: 1994 ident: 8 publication-title: Clin. Chem. doi: 10.1093/clinchem/40.10.1898 contributor: fullname: Miller J. J., Straub R. W., Jr., Valdes R., Jr.
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Snippet	There is an antiserum elicited by digoxin 3′-hemisuccinate–bovine serum albumin (BSA) conjugate possessing high specificity for digoxin. Our study focused on... There is an antiserum elicited by digoxin 3'-hemisuccinate-bovine serum albumin (BSA) conjugate possessing high specificity for digoxin. Our study focused on...
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SubjectTerms	Analysis Animals anti-digoxin antiserum Biological and medical sciences Cardiotonic agents Cardiovascular system Cattle digoxin Digoxin - analogs & derivatives Digoxin - analysis Digoxin - blood digoxin 3′-hemisuccinate–bovine serum albumin (BSA) conjugate General pharmacology Humans Immune Sera - analysis Immune Sera - blood Medical sciences Pharmacology. Drug treatments Radioimmunoassay - methods Radioimmunoassay - statistics & numerical data RIA Serum Albumin, Bovine - analysis
Title	Development of Radioimmunoassay for Measurement of Serum Digoxin in Digitalized Patients Using Novel Anti-digoxin Antiserum
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