Serological and Entomological Study of Dengue in Dang and Chitwan Districts of Nepal

• Published in: PloS one Vol. 11; no. 2; p. e0147953
• Main Authors: Shrestha, Rojina, Pant, Narayan Dutt, Gc, Ganga, Thapa, Srinivas, Neupane, Biswas, Shah, Yogendra, Gautam, Ishan, Pandey, Basu Dev
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01-02-2016; Public Library of Science (PLoS)
• Subjects: Adolescent; Adult; Aedes - growth & development; Aedes - physiology; Aedes aegypti; Aedes albopictus; Animals; Anorexia; Biology and Life Sciences; Breeding; Causes of; Chloride ions; Consent; Culicidae; Dengue; Dengue - blood; Dengue - epidemiology; Dengue fever; Disease; Distribution; Eating disorders; Ecology and Environmental Sciences; Ecosystem; Enzyme-linked immunosorbent assay; Epidemics; Fever; Headache; Hematology; Hospitals; Humans; Illnesses; Immunoglobulin M; Infections; Insect Vectors - growth & development; Ion concentration; Laboratories; Larva - growth & development; Leukopenia; Medical personnel; Medicine and Health Sciences; Middle Aged; Mosquitoes; Myalgia; Nausea; Nepal; Nepal - epidemiology; Pain; Patients; People and Places; pH effects; Physical Sciences; Public health; Skin; Surveillance; Surveys and Questionnaires; Thrombocytopenia; Tires; Vector-borne diseases; Vectors; Viral diseases; Viral infections; Viruses; Water; Workers; Young Adult; Nepal; India
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0147953

A descriptive cross-sectional study was carried out among 264 suspected dengue patients in two districts (Dang and Chitwan) of Nepal from June 2013 to November 2013. The anti-dengue IgM positivity was found to be (51/264)19.31% by capture ELISA, of which 21 (41.2%) were male and 30 (58.8%) were female. Symptoms of seropositive cases were fever, anorexia, nausea, headache, retro-orbital pain, skin rashes, and myalgia. Hematological features like thrombocytopenia and leucopenia were found to be significantly associated with the dengue fever (DF). Discarded tires were found as the commonest breeding habitats for the dengue vectors. Higher sero-positivity was recorded from the area having higher Breteau index (BI). The pH, chloride ion concentration and the salinity of the water from breeding habitats were found to be ranging from 6.9±0.82 to 8, 103.33±17.52 mg/L to 140.65 mg/L, and 0.19±0.032 ppt to 0.25 ppt respectively. This study may be helpful for the health authorities and public health workers for early diagnosis of DF and for the improved preventive measures to be adopted in the epidemic and possible epidemic areas.