Soluble adenylyl cyclase: A novel player in cardiac hypertrophy induced by isoprenaline or pressure overload

• Published in: PloS one Vol. 13; no. 2; p. e0192322
• Main Authors: Schirmer, Ilona, Bualeong, Tippaporn, Budde, Heidi, Cimiotti, Diana, Appukuttan, Avinash, Klein, Nicole, Steinwascher, Philip, Reusch, Peter, Mügge, Andreas, Meyer, Rainer, Ladilov, Yury, Jaquet, Kornelia
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21-02-2018; Public Library of Science (PLoS)
• Subjects: Actin; Adenylate cyclase; Adrenergic receptors; Analysis; Animal tissues; Aorta; Apoptosis; Atrial natriuretic peptide; Bicarbonates; Biology and Life Sciences; Calcium; Carbonates; Cardiac muscle; Cardiology; Cardiomyocytes; Cell growth; Cell size; Cyclic AMP response element-binding protein; Cytosol; Deoxyribonucleic acid; Development and progression; DNA; Gene expression; Health aspects; Heart; Heart diseases; Heart enlargement; Heart failure; Hemodynamics; Hypertrophy; Kinases; Laboratory animals; Medicine and Health Sciences; Mice; Mitochondria; Muscles; Nuclei; Pharmacology; Phosphorylation; Physiological aspects; Physiology; Pressure; Prostate; Proteins; Research and Analysis Methods; Ribonucleic acid; RNA; Rodents; Stimulation; Transcription factors; Germany
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0192322

In contrast to the membrane bound adenylyl cyclases, the soluble adenylyl cyclase (sAC) is activated by bicarbonate and divalent ions including calcium. sAC is located in the cytosol, nuclei and mitochondria of several tissues including cardiac muscle. However, its role in cardiac pathology is poorly understood. Here we investigate whether sAC is involved in hypertrophic growth using two different model systems.
In isolated adult rat cardiomyocytes hypertrophy was induced by 24 h β1-adrenoceptor stimulation using isoprenaline (ISO) and a β2-adrenoceptor antagonist (ICI118,551). To monitor hypertrophy cell size along with RNA/DNA- and protein/DNA ratios as well as the expression level of α-skeletal actin were analyzed. sAC activity was suppressed either by treatment with its specific inhibitor KH7 or by knockdown. Both pharmacological inhibition and knockdown blunted hypertrophic growth and reduced expression levels of α-skeletal actin in ISO/ICI treated rat cardiomyocytes. To analyze the underlying cellular mechanism expression levels of phosphorylated CREB, B-Raf and Erk1/2 were examined by western blot. The results suggest the involvement of B-Raf, but not of Erk or CREB in the pro-hypertrophic action of sAC. In wild type and sAC knockout mice pressure overload was induced by transverse aortic constriction. Hemodynamics, heart weight and the expression level of the atrial natriuretic peptide were analyzed. In accordance, transverse aortic constriction failed to induce hypertrophy in sAC knockout mice. Mechanistic analysis revealed a potential role of Erk1/2 in TAC-induced hypertrophy.
Soluble adenylyl cyclase might be a new pivotal player in the cardiac hypertrophic response either to long-term β1-adrenoceptor stimulation or to pressure overload.