Historical Invasion Records Can Be Misleading: Genetic Evidence for Multiple Introductions of Invasive Raccoons (Procyon lotor) in Germany

• Published in: PloS one Vol. 10; no. 5; p. e0125441
• Main Authors: Fischer, Mari L, Hochkirch, Axel, Heddergott, Mike, Schulze, Christoph, Anheyer-Behmenburg, Helena E, Lang, Johannes, Michler, Frank-Uwe, Hohmann, Ulf, Ansorge, Hermann, Hoffmann, Lothar, Klein, Roland, Frantz, Alain C
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 06-05-2015; Public Library of Science (PLoS)
• Subjects: Adaptation; Animals; Biodiversity; Biogeography; Biological evolution; Clustering; Common raccoon; Ecological monitoring; Ecosystem; Evolution; Founder Effect; Genetic aspects; Genetic diversity; Genetic Variation - genetics; Genetics, Population; Germany; Haplotypes; Historic artifacts; Introduced Species; Invasive species; Microsatellite Repeats - genetics; Microsatellites (Genetics); Mitochondrial DNA; Nonnative species; Population; Population genetics; Population number; Pressure effects; Procyon lotor; Raccoons - genetics; Range extension; Species diversity; Subpopulations; United States; United States; Germany
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0125441

Biological invasions provide excellent study systems to understand evolutionary, genetic and ecological processes during range expansions. There is strong evidence for positive effects of high propagule pressure and the associated higher genetic diversity on invasion success, but some species have become invasive despite small founder numbers. The raccoon (Procyon lotor) is often considered as a typical example for such a successful invasion resulting from a small number of founders. The species' largest non-native population in Germany is commonly assumed to stem from a small number of founders and two separate founding events in the 1930s and 1940s. In the present study we analyzed 407 raccoons at 20 microsatellite loci sampled from the invasive range in Western Europe to test if these assumptions are correct. Contrary to the expectations, different genetic clustering methods detected evidence for at least four independent introduction events that gave rise to genetically differentiated subpopulations. Further smaller clusters were either artifacts or resulted from founder events at the range margin and recent release of captive individuals. We also found genetic evidence for on-going introductions of individuals. Furthermore a novel randomization process was used to determine the potential range of founder population size that would suffice to capture all the alleles present in a cluster. Our results falsify the assumption that this species has become widespread and abundant despite being genetically depauperate and show that historical records of species introductions may be misleading.