Genome analysis of environmental and clinical P. aeruginosa isolates from sequence type-1146

• Published in: PloS one Vol. 9; no. 10; p. e107754
• Main Authors: Sánchez, David, Gomila, Margarita, Bennasar, Antonio, Lalucat, Jorge, García-Valdés, Elena
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 15-10-2014; Public Library of Science (PLoS)
• Subjects: Adaptation, Physiological - drug effects; Anti-Bacterial Agents - therapeutic use; Antibiotics; Bacteriophages - genetics; Bacteriophages - metabolism; Bioinformatics; Biology and Life Sciences; CRISPR; Cystic fibrosis; Drug Resistance, Bacterial - drug effects; Drug Resistance, Bacterial - genetics; Ecosystems; Evolution (Biology); Genes; Genome, Bacterial; Genomes; Genomics; Humans; Infectivity; Mutation; Nucleotide sequence; Pathogenicity; Pathogens; Phages; Pseudomonas; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - genetics; Spacer region; Studies; Majorca; Spain
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0107754

The genomes of Pseudomonas aeruginosa isolates of the new sequence type ST-1146, three environmental (P37, P47 and P49) and one clinical (SD9) isolates, with differences in their antibiotic susceptibility profiles have been sequenced and analysed. The genomes were mapped against P. aeruginosa PAO1-UW and UCBPP-PA14. The allelic profiles showed that the highest number of differences were in "Related to phage, transposon or plasmid" and "Secreted factors" categories. The clinical isolate showed a number of exclusive alleles greater than that for the environmental isolates. The phage Pf1 region in isolate SD9 accumulated the highest number of nucleotide substitutions. The ORF analysis of the four genomes assembled de novo indicated that the number of isolate-specific genes was higher in isolate SD9 (132 genes) than in isolates P37 (24 genes), P47 (16 genes) and P49 (21 genes). CRISPR elements were found in all isolates and SD9 showed differences in the spacer region. Genes related to bacteriophages F116 and H66 were found only in isolate SD9. Genome comparisons indicated that the isolates of ST-1146 are close related, and most genes implicated in pathogenicity are highly conserved, suggesting a genetic potential for infectivity in the environmental isolates similar to the clinical one. Phage-related genes are responsible of the main differences among the genomes of ST-1146 isolates. The role of bacteriophages has to be considered in the adaptation processes of isolates to the host and in microevolution studies.