Sarcoidosis and tuberculosis cytokine profiles: indistinguishable in bronchoalveolar lavage but different in blood

• Published in: PloS one Vol. 7; no. 7; p. e38083
• Main Authors: Thillai, Muhunthan, Eberhardt, Christian, Lewin, Alex M, Potiphar, Lee, Hingley-Wilson, Suzie, Sridhar, Saranya, Macintyre, Jonathan, Kon, Onn Min, Wickremasinghe, Melissa, Wells, Athol, Weeks, Mark E, Mitchell, Donald, Lalvani, Ajit
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16-07-2012; Public Library of Science (PLoS)
• Subjects: Adolescent; Adult; Aged; Alveoli; Arthritis; B cells; Biology; Biomarkers - blood; Bronchoalveolar Lavage Fluid - chemistry; Bronchoalveolar Lavage Fluid - immunology; Bronchus; Critical care; Cytokines; Cytokines - blood; Demography; Development and progression; Diagnosis, Differential; Differentiation; Enzymes; Female; Health care; Heart; Hospitals; Humans; Immunology; Infections; Inflammation; Interleukin 4; Interleukins; Lung diseases; Lymphocytes T; Male; Medical diagnosis; Medicine; Middle Aged; Model accuracy; Regression analysis; Regression models; Sarcoidosis; Sarcoidosis, Pulmonary - blood; Sarcoidosis, Pulmonary - diagnosis; Sarcoidosis, Pulmonary - immunology; Scleroderma; Studies; Tuberculosis; Tuberculosis, Pulmonary - blood; Tuberculosis, Pulmonary - diagnosis; Tuberculosis, Pulmonary - immunology; Young Adult; United Kingdom > UK
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0038083

The clinical, radiological and pathological similarities between sarcoidosis and tuberculosis can make disease differentiation challenging. A complicating factor is that some cases of sarcoidosis may be initiated by mycobacteria. We hypothesised that immunological profiling might provide insight into a possible relationship between the diseases or allow us to distinguish between them.
We analysed bronchoalveolar lavage (BAL) fluid in sarcoidosis (n = 18), tuberculosis (n = 12) and healthy volunteers (n = 16). We further investigated serum samples in the same groups; sarcoidosis (n = 40), tuberculosis (n = 15) and healthy volunteers (n = 40). A cross-sectional analysis of multiple cytokine profiles was performed and data used to discriminate between samples.
We found that BAL profiles were indistinguishable between both diseases and significantly different from healthy volunteers. In sera, tuberculosis patients had significantly lower levels of the Th2 cytokine interleukin-4 (IL-4) than those with sarcoidosis (p = 0.004). Additional serum differences allowed us to create a linear regression model for disease differentiation (within-sample accuracy 91%, cross-validation accuracy 73%).
These data warrant replication in independent cohorts to further develop and validate a serum cytokine signature that may be able to distinguish sarcoidosis from tuberculosis. Systemic Th2 cytokine differences between sarcoidosis and tuberculosis may also underly different disease outcomes to similar respiratory stimuli.