Analysis of biliary MICRObiota in hepatoBILIOpancreatic diseases compared to healthy people [MICROBILIO]: Study protocol

• Published in: PloS one Vol. 15; no. 11; p. e0242553
• Main Authors: Nascimento, Fernanda Sayuri do, Suzuki, Milena Oliveira, Taba, João Victor, de Mattos, Vitoria Carneiro, Pipek, Leonardo Zumerkorn, D'Albuquerque, Eugênia Machado Carneiro, Iuamoto, Leandro, Meyer, Alberto, Andraus, Wellington, Pinho, João Renato Rebello, de Moura, Eduardo Guimarães Hourneaux, Setubal, João Carlos, Carneiro-D'Albuquerque, Luiz Augusto
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19-11-2020; Public Library of Science (PLoS)
• Subjects: Adult; Antibiotics; Bacteria; Bile; Bile - microbiology; Biliary tract; Biology and Life Sciences; Biomarkers; Case-Control Studies; Cholangiopancreatography, Endoscopic Retrograde; Colorectal cancer; Composition; Deoxyribonucleic acid; Diagnosis; Digestive system diseases; Digestive System Diseases - microbiology; Digestive tract; Disease; Diseases; DNA; DNA sequencing; DNA, Bacterial - genetics; Female; Gastroenterology; Gastrointestinal cancer; Gastrointestinal tract; Gene sequencing; Genetic aspects; Genomes; Health aspects; Health care; Homeostasis; Hospitals; Humans; Liver Transplantation; Living Donors; Male; Medical prognosis; Medicine; Medicine and Health Sciences; Metabolism; Metabolites; Metagenome; Microbiomes; Microbiota; Microbiota (Symbiotic organisms); Microbiota - genetics; Middle Aged; Nucleotide sequence; Pancreatic cancer; Polyamines; Protocol (computers); Registered Report Protocol; Research and analysis methods; Ribotyping; RNA sequencing; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; rRNA 16S; Transplantation; Young Adult; Brazil
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0242553

The performance of the microbiota is observed in several digestive tract diseases. Therefore, reaching the biliary microbiota may suggest ways for studies of biomarkers, diagnoses, tests and therapies in hepatobiliopancreatic diseases.
Bile samples will be collected in endoscopic retrograde cholangiopancreatography patients (case group) and living liver transplantation donors (control group). We will characterize the microbiome based on two types of sequence data: the V3/V4 regions of the 16S ribosomal RNA (rRNA) gene and total shotgun DNA. For 16S sequencing data a standard 16S processing pipeline based on the Amplicon Sequence Variant concept and the qiime2 software package will be employed; for shotgun data, for each sample we will assemble the reads and obtain and analyze metagenome-assembled genomes.
The primary expected results of the study is to characterize the specific composition of the biliary microbiota in situations of disease and health. In addition, it seeks to demonstrate the existence of changes in the case of illness and also possible disease biomarkers, diagnosis, interventions and therapies in hepatobiliopancreatic diseases.
NCT04391426. Registered 18 May 2020, https://clinicaltrials.gov/ct2/show/NCT04391426.