SREBF1c and SREBF2 gene polymorphisms are associated with acute coronary syndrome and blood lipid levels in Mexican population

• Published in: PloS one Vol. 14; no. 9; p. e0222017
• Main Authors: Vargas-Alarcon, Gilberto, Gonzalez-Pacheco, Hector, Perez-Mendez, Oscar, Posadas-Sanchez, Rosalinda, Cardoso-Saldaña, Guillermo, Ramirez-Bello, Julian, Escobedo, Galileo, Nieto-Lima, Betzabe, Fragoso, Jose Manuel
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 06-09-2019; Public Library of Science (PLoS)
• Subjects: Acute Coronary Syndrome - blood; Acute Coronary Syndrome - genetics; Acute coronary syndromes; Aged; Analysis; Arteriosclerosis; Atherosclerosis; Atherosclerotic plaque; Biology and Life Sciences; Biosynthesis; Blood lipids; Cardiology; Cardiovascular disease; Case-Control Studies; Cholesterol; Chromosomes; Control methods; Coronary heart disease; Coronary vessels; Diabetes; DNA binding proteins; Endocrinology; Exonuclease; Fatty acids; Female; Gene Frequency; Genes; Genetic polymorphisms; Genetic research; Genetic susceptibility; Genotype; Genotypes; Health care; Heredity; Humans; Insulin resistance; Kinases; Linkage Disequilibrium; Lipids; Lipids - blood; Lipoproteins; Liver diseases; Male; Mathematical analysis; Medical research; Medicine and Health Sciences; Metabolic disorders; Mexico; Middle Aged; Molecular biology; Nucleotides; People and places; Polymorphism; Polymorphism, Single Nucleotide; Regulation; Regulatory sequences; Risk; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Statistical analysis; Sterol Regulatory Element Binding Protein 1 - genetics; Sterol Regulatory Element Binding Protein 2 - genetics; Sterols; Studies; Transcription (Genetics); Transcription factors; Triglycerides; Type 2 diabetes; Mexico
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0222017

It has recently been reported that the sterol regulatory element-binding transcription factors (SREBF-1c, and -2) contribute to the variation in the plasma lipids levels, which have an important role in the atherosclerotic plaque development. The aim of the present study was to evaluate whether the SREBF1c and SREBF2 gene single nucleotide polymorphisms (SNPs) are associated with plasma lipids levels and ACS susceptibility in a case-control association study.
A case-control study was carried out in 625 patients with ACS (82% men and 18% women, with a mean age of 57.97 ± 10.5 years) and 700 healthy controls (66% men and 34% women, with a mean age of 54.37 ± 7.65 years). The sample size was calculated for a statistical power of 80%. We genotyped three SREBF1c (rs2297508, rs11656665 and rs11868035) and three SREBF2 (rs2267439, rs2267443, and rs2228314) gene polymorphisms by 5' exonuclease TaqMan assays. The associations were evaluated by logistic regression under the co-dominant, dominant, recessive, over-dominant and additive inheritance models. The contribution of the genotypes on the plasma lipids levels was evaluated by Student's t-test.
Under different models, the SREBF1c rs2297508 (OR = 1.50, pCRes = 0.03), SREBF1c rs11656665 (OR = 1.35, pCDom = 0.02 and OR = 1.26, pCAdd = 0.02) and SREBF2 rs2228314 (OR = 1.78, pCRes = 0.03, OR = 1.27, pCAdd = 0.04) SNPs were associated with higher risk of ACS. On the other hand, the SREBF1c rs11868035 SNP was associated with lower risk of ACS (OR = 0.49, pCCo-dom = 0.001, OR = 0.66, pCDom = 0.003, OR = 0.57, PRes = 0.003 and OR = 0.71, pCAdd = 0.001). There was a statistically significant association of both SREBF1c rs11656665 and rs11868035 polymorphisms with plasma triglyceride levels.
In summary, our data suggest the association of the SREBF1c and SREBF2 SNPs with risk of developing ACS and with triglyceride levels in a Mexican population.