QTL analysis reveals quantitative resistant loci for Phytophthora infestans and Tecia solanivora in tetraploid potato (Solanum tuberosum L.)

• Published in: PloS one Vol. 13; no. 7; p. e0199716
• Main Authors: Santa, Juan David, Berdugo-Cely, Jhon, Cely-Pardo, Liliana, Soto-Suárez, Mauricio, Mosquera, Teresa, Galeano M, Carlos H
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 06-07-2018; Public Library of Science (PLoS)
• Subjects: Animals; Autotetraploid; Biology and Life Sciences; Blight; Breeding; Chromosome Mapping; CRISPR; Crop diseases; Crops; Cultivars; Disease Resistance - genetics; Gelechiidae; Gene mapping; Genes; Genetic aspects; Genetic polymorphisms; Genomes; Genomics; Genotype & phenotype; Genotypes; Heterozygosity; Immunity, Innate - genetics; Immunity, Innate - immunology; Late blight; Lepidoptera; Molecular chains; Moths - physiology; Phenotype; Phytophthora diseases; Phytophthora infestans; Phytophthora infestans - genetics; Phytophthora infestans - parasitology; Plant breeding; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - parasitology; Plant location; Plant Proteins - genetics; Polymorphism, Single Nucleotide; Population; Potatoes; Progeny; Quantitative Trait Loci; Research and Analysis Methods; Single-nucleotide polymorphism; Solanum; Solanum tuberosum; Solanum tuberosum - genetics; Solanum tuberosum - growth & development; Solanum tuberosum - immunology; Solanum tuberosum - parasitology; Tetraploidy; Vegetables; Ecuador; Colombia
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0199716

Late blight and Guatemalan potato tuber moth caused by Phytophthora infestans and Tecia solanivora, respectively, are major phytosanitary problems on potato crops in Colombia and Ecuador. Hence, the development of resistant cultivars is an alternative for their control. However, breeding initiatives for durable resistance using molecular tools are limited due to the genome complexity and high heterozygosity in autotetraploid potatoes. To contribute to a better understanding of the genetic basis underlying the resistance to P. infestans and T. solanivora in potato, the aim of this study was to identify QTLs for resistance to P. infestans and T. solanivora using a F1 tetraploid potato segregant population for both traits. Ninety-four individuals comprised this population. Parent genotypes and their progeny were genotyped using SOLCAP 12K potato array. Forty-five percent of the markers were polymorphic. A genetic linkage map was built with a length of 968.4 cM and 1,287 SNPs showing good distribution across the genome. Severity and incidence were evaluated in two crop cycles for two years. QTL analysis revealed six QTLs linked to P. infestans, four of these related to previous QTLs reported, and two novel QTLs (qrAUDPC-3 and qrAUDPC-8). Fifteen QTLs were linked to T. solanivora, being qIPC-6 and qOPA-6.1, and qIPC-10 and qIPC-10.1 stable in two different trials. This study is one of the first to identify QTLs for T. solanivora. As the population employed is a breeding population, results will contribute significantly to breeding programs to select resistant plant material, especially in countries where P. infestans and T. solanivora limit potato production.