Development and validation of Burkholderia pseudomallei-specific real-time PCR assays for clinical, environmental or forensic detection applications

The bacterium Burkholderia pseudomallei causes melioidosis, a rare but serious illness that can be fatal if untreated or misdiagnosed. Species-specific PCR assays provide a technically simple method for differentiating B. pseudomallei from near-neighbor species. However, substantial genetic diversit...

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Bibliographic Details
Published in:	PloS one Vol. 7; no. 5; p. e37723
Main Authors:	Price, Erin P, Dale, Julia L, Cook, James M, Sarovich, Derek S, Seymour, Meagan L, Ginther, Jennifer L, Kaufman, Emily L, Beckstrom-Sternberg, Stephen M, Mayo, Mark, Kaestli, Mirjam, Glass, Mindy B, Gee, Jay E, Wuthiekanun, Vanaporn, Warner, Jeffrey M, Baker, Anthony, Foster, Jeffrey T, Tan, Patrick, Tuanyok, Apichai, Limmathurotsakul, Direk, Peacock, Sharon J, Currie, Bart J, Wagner, David M, Keim, Paul, Pearson, Talima
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 18-05-2012 Public Library of Science (PLoS)
Subjects:	Assaying Bacteria Biodiversity Biology Burkholderia Burkholderia mallei Burkholderia pseudomallei Burkholderia pseudomallei - genetics Deoxyribonucleic acid Disease control Disease prevention DNA Forensic engineering Forensic science Genetic diversity Genetics Genomics Identification Immunology Linearity Medical research Medicine Melioidosis Melioidosis - diagnosis Melioidosis - microbiology Polymerase chain reaction Polymorphism, Single Nucleotide - genetics Pseudomonas Quality control Quantitation Real-Time Polymerase Chain Reaction - methods Recombination Ruggedness Scientific research Selectivity Sequence Analysis, DNA Soil microorganisms Species Species diversity Species Specificity Atlanta Georgia Georgia Thailand United States > US Arizona Bangkok Thailand
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