Periostin secreted by activated fibroblasts in idiopathic pulmonary fibrosis promotes tumorigenesis of non-small cell lung cancer

• Published in: Scientific reports Vol. 11; no. 1; pp. 21114 - 15
• Main Authors: Yamato, Hiroyuki, Kimura, Kenji, Fukui, Eriko, Kanou, Takashi, Ose, Naoko, Funaki, Soichiro, Minami, Masato, Shintani, Yasushi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 26-10-2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/67/1612; 631/67/327; A549 Cells; Carcinogenesis - genetics; Carcinogenesis - metabolism; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - metabolism; Cell Adhesion Molecules - biosynthesis; Cell Adhesion Molecules - genetics; Cell proliferation; Extracellular matrix; Female; Fibroblasts; Fibroblasts - metabolism; Fibrosis; Humanities and Social Sciences; Humans; Idiopathic Pulmonary Fibrosis - genetics; Idiopathic Pulmonary Fibrosis - metabolism; Lung cancer; Lung diseases; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Male; Matrix protein; multidisciplinary; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; Non-small cell lung carcinoma; Pulmonary fibrosis; Science; Science (multidisciplinary); Small cell lung carcinoma; Tumorigenesis
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-00717-5

Non-small cell lung cancer (NSCLC) patients with idiopathic pulmonary fibrosis (IPF) show poor prognosis. Periostin is an extracellular matrix protein highly expressed in the lung tissues of IPF. This study aimed to investigate the possibility that periostin secreted by fibroblasts derived from IPF lung might affect proliferation of NSCLC cells. Periostin was more highly expressed and secreted by fibroblasts from diseased human lung with IPF (DIPF) than by normal human lung fibroblasts (NHLF). Cocultivation of NSCLC cells with conditioned media (CM) from DIPF increased proliferation of NSCLC cells through pErk signaling, with this proliferation attenuated by periostin-neutralizing antibodies. Knockdown of integrin β3, a subunit of the periostin receptor, in NSCLC cells suppressed proliferation of NSCLC cells promoted by recombinant human periostin and CM of DIPF. On in vivo examination, DIPF promoted tumor progression more than NHLF, and knockdown of integrin β3 in NSCLC cells suppressed tumor progression promoted by DIPF. Fibroblasts derived from surgical specimens from IPF patients also increased secretion of periostin compared to those from non-IPF patients. Periostin secreted from IPF-activated fibroblasts plays critical roles in the proliferation of NSCLC cells. The present study provides a solid basis for considering periostin-targeted therapy for NSCLC patients with IPF.