A Rapid Derivative Spectrophotometric Method for Simultaneous Determination of Naphazoline and Antazoline in Eye Drops
A zero-crossing first-derivative spectrophotometric method is applied for the simultaneous determination of naphazoline hydrochloride and antazoline phosphate in eye drops. The measurements were carried out at wavelengths of 225 and 252 nm for naphazoline hydrochloride and antazoline phosphate, resp...
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| Published in: | Chemical & Pharmaceutical Bulletin Vol. 54; no. 1; pp. 119 - 122 |
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2006
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| Abstract | A zero-crossing first-derivative spectrophotometric method is applied for the simultaneous determination of naphazoline hydrochloride and antazoline phosphate in eye drops. The measurements were carried out at wavelengths of 225 and 252 nm for naphazoline hydrochloride and antazoline phosphate, respectively. The method was found to be linear (r2>0.999) in the range of 0.2—1 μg/ml for naphazoline hydrochloride in the presence of 5 μg/ml antazoline phosphate at 225 nm. The same linear correlation (r2>0.999) was obtained in the range of 1—10 μg/ml of antazoline phosphate in the presence of 0.5 μg/ml of naphazoline hydrochloride at 252 nm. The limit of determination was 0.2 μg/ml and 1 μg/ml for naphazoline hydrochloride and antazoline phosphate, respectively. The method was successfully used for simultaneous analysis of naphazoline hydrochloride and antazoline phosphate in eye drops without any interference from excipients and prior separation before analysis. |
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| AbstractList | A zero-crossing first-derivative spectrophotometric method is applied for the simultaneous determination of naphazoline hydrochloride and antazoline phosphate in eye drops. The measurements were carried out at wavelengths of 225 and 252 nm for naphazoline hydrochloride and antazoline phosphate, respectively. The method was found to be linear (r2>0.999) in the range of 0.2—1 μg/ml for naphazoline hydrochloride in the presence of 5 μg/ml antazoline phosphate at 225 nm. The same linear correlation (r2>0.999) was obtained in the range of 1—10 μg/ml of antazoline phosphate in the presence of 0.5 μg/ml of naphazoline hydrochloride at 252 nm. The limit of determination was 0.2 μg/ml and 1 μg/ml for naphazoline hydrochloride and antazoline phosphate, respectively. The method was successfully used for simultaneous analysis of naphazoline hydrochloride and antazoline phosphate in eye drops without any interference from excipients and prior separation before analysis. A zero-crossing first-derivative spectrophotometric method is applied for the simultaneous determination of naphazoline hydrochloride and antazoline phosphate in eye drops. The measurements were carried out at wavelengths of 225 and 252 nm for naphazoline hydrochloride and antazoline phosphate, respectively. The method was found to be linear (γ2>0.999) in the range of 0.2-1μg/ml for naphazoline hydrochloride in the presence of 5μg/ml antazoline phosphate at 225 nm. The same linear correlation (γ2>0.999) was obtained in the range of 110μg/ml of antazoline phosphate in the presence of 0.5μg/ml of, naphazoline hydrochloride at 252 nm. The limit of determination was 0.2μg/ml and 1μg/ml for naphazoline hydrochloride and antazoline phosphate, respectively. The method was successfully used for simultaneous analysis of naphazoline hydrochloride and antazoline phosphate in eye drops without any interference from excipients and prior separation before analysis. A zero-crossing first-derivative spectrophotometric method is applied for the simultaneous determination of naphazoline hydrochloride and antazoline phosphate in eye drops. The measurements were carried out at wavelengths of 225 and 252 nm for naphazoline hydrochloride and antazoline phosphate, respectively. The method was found to be linear (r2>0.999) in the range of 0.2-1 microg/ml for naphazoline hydrochloride in the presence of 5 microg/ml antazoline phosphate at 225 nm. The same linear correlation (r2>0.999) was obtained in the range of 1-10 microg/ml of antazoline phosphate in the presence of 0.5 microg/ml of naphazoline hydrochloride at 252 nm. The limit of determination was 0.2 microg/ml and 1 microg/ml for naphazoline hydrochloride and antazoline phosphate, respectively. The method was successfully used for simultaneous analysis of naphazoline hydrochloride and antazoline phosphate in eye drops without any interference from excipients and prior separation before analysis. A zero-crossing first-derivative spectrophotometric method is applied for the simultaneous determination of naphazoline hydrochloride and antazoline phosphate in eye drops. The measurements were carried out at wavelengths of 225 and 252 nm for naphazoline hydrochloride and antazoline phosphate, respectively. The method was found to be linear (r2>0.999) in the range of 0.2-1 microg/ml for naphazoline hydrochloride in the presence of 5 microg/ml antazoline phosphate at 225 nm. The same linear correlation (r2>0.999) was obtained in the range of 1-10 microg/ml of antazoline phosphate in the presence of 0.5 microg/ml of naphazoline hydrochloride at 252 nm. The limit of determination was 0.2 microg/ml and 1 microg/ml for naphazoline hydrochloride and antazoline phosphate, respectively. The method was successfully used for simultaneous analysis of naphazoline hydrochloride and antazoline phosphate in eye drops without any interference from excipients and prior separation before analysis. |
| Author | Afshari, Alma Farsam, Hassan Souri, Effat Amanlou, Massoud |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/16394564$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1016/S0731-7085(01)00394-6 10.1016/S0731-7085(01)00627-6 10.1002/jps.2600680920 10.1016/S0731-7085(03)00347-9 10.1002/jps.2600670143 10.1016/S0731-7085(00)00596-3 10.1016/S0731-7085(01)00428-9 10.1111/j.2042-7158.1972.tb09050.x 10.1016/S0731-7085(01)00556-8 10.1016/S0731-7085(01)00664-1 10.1016/S0731-7085(03)00429-1 10.1016/S0731-7085(00)00600-2 10.1016/S0731-7085(02)00065-1 10.1016/S0731-7085(01)00388-0 10.1016/S0731-7085(01)00583-0 10.1016/S0731-7085(02)00095-X 10.1016/S0731-7085(01)00474-5 10.1002/jps.2600840422 |
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| SubjectTerms | Adrenergic alpha-Agonists - analysis Antazoline - analysis antazoline phosphate Anti-Allergic Agents - analysis Calibration derivative spectrophotometry eye drops Indicators and Reagents Naphazoline - analysis naphazoline hydrochloride Ophthalmic Solutions - analysis Reference Standards Reproducibility of Results Spectrophotometry, Ultraviolet |
| Title | A Rapid Derivative Spectrophotometric Method for Simultaneous Determination of Naphazoline and Antazoline in Eye Drops |
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