High level activity of 2',5'-oligoadenylate synthetase in dog serum

• Published in: Journal of Veterinary Medical Science Vol. 66; no. 6; pp. 721 - 724
• Main Authors: Iwata, A. (Nippon Inst. for Biological Science, Ome, Tokyo (Japan)), Yamamoto, A, Fujino, M, Sato, I, Hosokawa Kanai, T, Tuchiya, K, Ishihama, A, Sokawa, Y
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01-06-2004; Japan Science and Technology Agency
• Subjects: 2', 5'-oligoadenylate synthetase; 2',5'-Oligoadenylate Synthetase - blood; Animals; Antibodies, Monoclonal; BLOOD SERUM; Blotting, Western - veterinary; canine; Cats; Chromatography, DEAE-Cellulose - veterinary; DOGS; Dogs - blood; ENZYMES; Female; Guinea Pigs; INTERFERON; Rabbits; Radioimmunoassay - veterinary; serum
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.66.721

Most animal cells that are exposed to interferon (IFN) experience an increase in the activity of 2', 5'-oligoadenylate synthetase (OAS), which is an important effector of IFN's antiviral action. OAS activity has been widely used in clinical chemistry as an indicator of IFN activity. In this study, we found that OAS activity in canine serum is 46.0 ± 40.4 nmol/dl/hr, which is 10- to 100-fold higher than in other animals such as the cat (1.9 ± 2.1), rabbit (4.0 ± 1.1), and guinea pig (0.3 ± 0.6). The canine OAS protein was detected by Western blotting using a 68M-10 monoclonal anti-murine OAS antibody, and was found to be composed of at least three distinct molecular species of p40 class OAS. Among these, the 40 and 42 kDa components were determined to be the major species in serum and fibroblast cell lines, respectively.