Mutation D816V alters the internal structure and dynamics of c-KIT receptor cytoplasmic region: implications for dimerization and activation mechanisms

• Published in: PLoS computational biology Vol. 7; no. 6; p. e1002068
• Main Authors: Laine, Elodie, Chauvot de Beauchêne, Isaure, Perahia, David, Auclair, Christian, Tchertanov, Luba
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01-06-2011; PLOS; Public Library of Science (PLoS)
• Subjects: Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Benzamides; Biochemistry, Molecular Biology; Bioinformatics; Biology; Cancer; Catalytic Domain; Computer Science; Dimerization; Drug resistance; Drug Resistance, Neoplasm; Drug therapy; Enzymes; Gene mutations; Genetic aspects; Health aspects; Humans; Imatinib Mesylate; Kinases; Life Sciences; Molecular biology; Molecular Dynamics Simulation; Mutation; Physiological aspects; Piperazines - chemistry; Piperazines - pharmacology; Principal Component Analysis; Protein kinases; Protein Stability; Proteins; Proto-Oncogene Proteins c-kit - chemistry; Proto-Oncogene Proteins c-kit - genetics; Proto-Oncogene Proteins c-kit - metabolism; Pyrimidines - chemistry; Pyrimidines - pharmacology; Risk factors; Thermodynamics; France; tyrosine-kinase; D816V; molecular dynamics simulation; KIT; Catalytic Domain; Piperazines; Humans; Antineoplastic Agents; Drug Resistance, Neoplasm; Proto-Oncogene Proteins c-kit; Molecular Dynamics Simulation; Thermodynamics; Pyrimidines; Benzamides; Mutation; Protein Stability; Dimerization; Principal Component Analysis
• ISSN: 1553-7358; 1553-734X; 1553-7358
• DOI: 10.1371/journal.pcbi.1002068

The type III receptor tyrosine kinase (RTK) KIT plays a crucial role in the transmission of cellular signals through phosphorylation events that are associated with a switching of the protein conformation between inactive and active states. D816V KIT mutation is associated with various pathologies including mastocytosis and cancers. D816V-mutated KIT is constitutively active, and resistant to treatment with the anti-cancer drug Imatinib. To elucidate the activating molecular mechanism of this mutation, we applied a multi-approach procedure combining molecular dynamics (MD) simulations, normal modes analysis (NMA) and binding site prediction. Multiple 50-ns MD simulations of wild-type KIT and its mutant D816V were recorded using the inactive auto-inhibited structure of the protein, characteristic of type III RTKs. Computed free energy differences enabled us to quantify the impact of D816V on protein stability in the inactive state. We evidenced a local structural alteration of the activation loop (A-loop) upon mutation, and a long-range structural re-organization of the juxta-membrane region (JMR) followed by a weakening of the interaction network with the kinase domain. A thorough normal mode analysis of several MD conformations led to a plausible molecular rationale to propose that JMR is able to depart its auto-inhibitory position more easily in the mutant than in wild-type KIT and is thus able to promote kinase mutant dimerization without the need for extra-cellular ligand binding. Pocket detection at the surface of NMA-displaced conformations finally revealed that detachment of JMR from the kinase domain in the mutant was sufficient to open an access to the catalytic and substrate binding sites.