Influence of dietary n-3 fatty acids on macrophage glycerophospholipid molecular species and peptidoleukotriene synthesis

• Published in: Journal of lipid research Vol. 32; no. 7; pp. 1205 - 1213
• Main Authors: Chapkin, RS, Akoh, CC, Miller, CC
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01-07-1991; American Society for Biochemistry and Molecular Biology; Elsevier
• Subjects: ACEITES DE PESCADO; ACIDE GRAS INSATURE; ACIDOS GRASOS INSATURADOS; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; CORPS GRAS; DIETA; Dietary Fats, Unsaturated - pharmacology; Eicosapentaenoic Acid - pharmacology; FAGOCITOS; Female; FOSFOLIPIDOS; Fundamental and applied biological sciences. Psychology; GRASAS; HUILE DE POISSON; Leukotrienes - biosynthesis; LIPIDE; LIPIDOS; Lipids; Macrophages - chemistry; Macrophages - drug effects; Macrophages - metabolism; Mice; Mice, Inbred C57BL; Miscellaneous; Other biological molecules; PERITOINE; Peritoneal Cavity; PERITONEO; PHAGOCYTE; PHOSPHATIDE; Phosphatidic Acids - biosynthesis; Phosphatidic Acids - chemistry; RATON; REGIME ALIMENTAIRE; SOURIS; SRS-A - biosynthesis; Cell culture; Incorporation; Rodentia; Phospholipid; Biosynthesis; Vertebrata; Mammalia; Diet; Mouse; Radioimmunoassay; Unsaturated fatty acid; Biosynthesis precursor; Gas liquid chromatography; Macrophage
• ISSN: 0022-2275; 1539-7262
• DOI: 10.1016/S0022-2275(20)41983-2

The study examined the ability of dietary n-3 fatty acids to modify mouse peritoneal macrophage glycerophospholipid molecular species and peptidoleukotriene synthesis. After a 2-week feeding period, fish versus corn oil feeding significantly (P less than 0.01) lowered n-6 polyunsaturated fatty acid (PUFA) mol % levels, i.e., arachidonic acid (20:4n-6) in diacylphosphatidylserine (PtdSer), diacylphosphatidylinositol (PtdIns), diacylglycerophosphoethanolamine (PtdEtn), alkenylacylglycerophosphoethanolamine (PlsEtn), and diacylglycerophosphocholine (PtdCho). A notable exception was alkylacylglycerophosphocholine (PakCho), where only moderate decreases in 16:0-20:4n-6 and 18:0-20:4n-6 species were observed after fish oil supplementation. The predominant n-3 PUFA in macrophage phospholipid subclasses was docosapentaenoic acid (22:5n-3). The major n-3 species were 18:0-22:5n-3 in PtdIns, PtdSer, glycerophosphoethanolamines (EtnGpl) and 16:0-22:5n-3 in PtdCho and PlsEtn. The major n-3-containing species in PakCho were 16:0-20:5n-3 and 18:1-22:6n-3. These findings indicate that n-3 PUFA are differentially incorporated into macrophage phospholipid subclasses after dietary fish oil supplementation, and suggest that phospholipid remodeling enzymes selectively discriminate between substrates based on compatibility of sn-1 covalent linkage and the composition of the sn-1 and sn-2 aliphatic chains. Macrophage peptidoleukotriene synthesis was also strongly influenced after fish oil feeding; the LTC5/LTC4 ratio was significantly higher (P less than 0.01) in fish oil-fed animals than in corn oil-fed animals, 0.85 versus 0.01, respectively. These ratios were subsequently compared to phospholipid molecular species 20:5n-3/20:4n-6 ratios in order to determine potential sources of eicosanoid precursors.