Treponema pallidum subsp. pallidum with an Artificially impaired TprK antigenic variation system is attenuated in the Rabbit model of syphilis

• Published in: PLoS pathogens Vol. 19; no. 3; p. e1011259
• Main Authors: Romeis, Emily, Lieberman, Nicole A P, Molini, Barbara, Tantalo, Lauren C, Chung, Benjamin, Phung, Quynh, Avendaño, Carlos, Vorobieva, Anastassia, Greninger, Alexander L, Giacani, Lorenzo
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01-03-2023; Public Library of Science (PLoS)
• Subjects: Analysis; Animals; Annealing; Antibodies; Antigenic Variation - genetics; Antigens; Bacterial infections; Biology and Life Sciences; Care and treatment; Cloning; Development and progression; Gene conversion; Genomes; Globus pallidus; Growth rate; Health aspects; Immune clearance; Infections; Inoculum; Lymph nodes; Medicine and Health Sciences; Pathogens; Porins; Propagation; Rabbits; Recombination; Research and Analysis Methods; Scaffolding; Sexually transmitted diseases; STD; Suicide; Syphilis; Testing; Treponema; Treponema pallidum; Variation; Viral antibodies; Virulence; Virulence (Microbiology); United States
• ISSN: 1553-7374; 1553-7366; 1553-7374
• DOI: 10.1371/journal.ppat.1011259

The TprK protein of the syphilis agent, Treponema pallidum subsp. pallidum (T. pallidum), undergoes antigenic variation in seven discrete variable (V) regions via non-reciprocal segmental gene conversion. These recombination events transfer information from a repertoire of 53 silent chromosomal donor cassettes (DCs) into the single tprK expression site to continually generate TprK variants. Several lines of research developed over the last two decades support the theory that this mechanism is central to T. pallidum's ability for immune avoidance and persistence in the host. Structural and modeling data, for example, identify TprK as an integral outer membrane porin with the V regions exposed on the pathogen's surface. Furthermore, infection-induced antibodies preferentially target the V regions rather than the predicted β-barrel scaffolding, and sequence variation abrogates the binding of antibodies elicited by antigenically different V regions. Here, we engineered a T. pallidum strain to impair its ability to vary TprK and assessed its virulence in the rabbit model of syphilis.
A suicide vector was transformed into the wild-type (WT) SS14 T. pallidum isolate to eliminate 96% of its tprK DCs. The resulting SS14-DCKO strain exhibited an in vitro growth rate identical to the untransformed strain, supporting that the elimination of the DCs did not affect strain viability in absence of immune pressure. In rabbits injected intradermally with the SS14-DCKO strain, generation of new TprK sequences was impaired, and the animals developed attenuated lesions with a significantly reduced treponemal burden compared to control animals. During infection, clearance of V region variants originally in the inoculum mirrored the generation of antibodies to these variants, although no new variants were generated in the SS14-DCKO strain to overcome immune pressure. Naïve rabbits that received lymph node extracts from animals infected with the SS14-DCKO strain remained uninfected.
These data further support the critical role of TprK in T. pallidum virulence and persistence during infection.