Induction of growth inhibition and differentiation of human leukemia HL-60 cells by a Tunisian gerboui olive [Olea europaea] leaf extract

• Published in: Bioscience, biotechnology, and biochemistry Vol. 71; no. 5; pp. 1306 - 1312
• Main Authors: Abaza, L.(Biotechnology Center, Hammam Lif (Tunisia)), Talorete, T.P.N, Yamada, P, Kurita, Y, Zarrouk, M, Isoda, H
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01-05-2007; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Biological and medical sciences; CELL DIFFERENTIATION; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cell Survival - drug effects; DIFERENCIACION CELULAR; DIFFERENCIATION CELLULAIRE; DNA Fragmentation - drug effects; Ethanol - chemistry; EXTRACTOS; EXTRACTS; EXTRAIT; FEUILLE; Fundamental and applied biological sciences. Psychology; Gerboui olive leaf extract; growth inhibition; GROWTH INHIBITORS; HL-60 Cells; HOJAS; Humans; INHIBIDORES DEL CRECIMIENTO; INHIBITEUR DE CROISSANCE; LEAVES; LEUCEMIA; LEUCEMIE; LEUKAEMIA; Leukemia - drug therapy; Nitroblue Tetrazolium - metabolism; Olea - chemistry; Olea - classification; OLEA EUROPAEA; Plant Extracts - pharmacology; Plant Leaves - chemistry; Tunisia; Tunisia; Human; Growth; Induction; Extract; Plant leaf; growth inhibition; Cell differentiation; Olive; Inhibition; HL-60 cells; Differentiation; Gerboui olive leaf extract; Tumor cell
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.60716

Cancer protection associated with the consumption of olive products is well established, but not for leukemia. The protective effects of olive (Olea europaea L.) leaves were investigated by incubating human promyelocytic leukemia HL-60 cells with olive leaf extracts (OLEs) from seven principal Tunisian olive varieties, namely, Chemchali, Chemlali, Chetoui, Gerboui, Sayali, Zalmati and Zarrazi. The results showed significant growth inhibition of HL-60 cells incubated for 48 h with a 100-fold dilution of each OLE which had been obtained by incubating 10 g of dried leaves in 100 ml of 70% ethanol for one week with subsequent ultrafiltration. DNA fragmentation was observed in the cells incubated for 19 h with a 100-fold dilution of the Chemchali, Chemlali and Zalmati extracts. The results of a nitroblue tetrazolium (NBT) assay revealed NBT reduction, a differentiation marker, by the OLE-treated cells after an overnight incubation. The Gerboui extract showed the highest NBT reduction ability at more than 90%. An HPLC analysis revealed the presence of apigenin 7-glucoside in the extract, which was found in subsequent experiments to be responsible for the Gerboui extract-mediated cell differentiation.