Genetic mapping and characterization of sorghum and related crops by means of maize DNA probes

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 87; no. 11; pp. 4251 - 4255
• Main Authors: Hulbert, S.H. (Purdue University, West Lafayette, IN), Richter, T.E, Axtell, J.D, Bennetzen, J.L
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01-06-1990; National Acad Sciences
• Subjects: ADN; Agronomy. Soil science and plant productions; Biological and medical sciences; Blotting, Southern; CHROMOSOME; Chromosome Mapping; CHROMOSOMES; Classical genetics, quantitative genetics, hybrids; Corn; CROMOSOMAS; DNA; DNA Probes; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genetic hybridization; Genetic Linkage; Genetic loci; GENETIC VARIATION; Genetics of eukaryotes. Biological and molecular evolution; GENOMAS; GENOME; GENOMES; Genomics; HORDEUM VULGARE; INSTRUMENT; INSTRUMENTOS; INSTRUMENTS; LINKAGE; Linkage groups; LOCI; LOCUS; PENNISETUM; Plants - genetics; POLIMORFISMO; POLYMORPHISM; Polymorphism, Restriction Fragment Length; POLYMORPHISME; Pteridophyta, spermatophyta; SACCHARUM; Sequence Homology, Nucleic Acid; SETARIA (GRAMINEA); SETARIA (GRAMINEE); SETARIA (GRASS); SORGHUM; Species Specificity; VARIACION GENETICA; VARIATION GENETIQUE; Vegetals; ZEA MAYS; Zea mays - genetics; Genetic mapping; Monocotyledones; Genome organization; Sorghum bicolor; Restriction fragment length polymorphism; Gramineae; DNA; Linkage group; Angiospermae; Spermatophyta; Molecular probe; Molecular cloning
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.87.11.4251

Cloned DNA fragments from 14 characterized maize genes and 91 random fragments used for genetic mapping in maize were tested for their ability to hybridize and detect restriction fragment length polymorphisms in sorghum and other related crop species. Most DNA fragments tested hybridized strongly to DNA from sorghum, foxtail millet, Johnsongrass, and sugarcane. Hybridization to pearl millet DNA was generally weaker, and only a few probes hybridized to barley DNA under the conditions used. Patterns of hybridization of low-copy sequences to maize and sorghum DNA indicated that the two genomes are very similar. Most probes detected two loci in maize; these usually detected two loci in sorghum. Probes that detected one locus in raise generally detected a single locus in sorghum. However, maize repetitive DNA sequences present on some of the genomic clones did not hybridize to sorghum DNA. Most of the probes tested detected polymorphisms among a group of seven diverse sorghum lines tested; over one-third of the probes detected polymorphism in a single F2population from two of these lines. Cosegregation analysis of 55 F2individuals enabled several linkage groups to be constructed and compared with the linkage relationships of the same loci in maize. The linkage relationships of the polymorphic loci in the two species were usually conserved, but several rearrangements were detected.