Methodology for Y Chromosome Capture: A complete genome sequence of  Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads

• Published in: Scientific reports Vol. 8; no. 1; pp. 9436 - 13
• Main Authors: Alvarez-Cubero, M. J., Santiago, O., Martínez-Labarga, C., Martínez-García, B., Marrero- Díaz, R., Rubio-Roldan, A., Pérez-Gutiérrez, A. M., Carmona-Saez, P., Lorente, J. A., Martinez-Gonzalez, L. J.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21-06-2018; Nature Publishing Group; Nature Portfolio
• Subjects: 13/31; 14/32; 14/63; 45/22; 45/23; 631/114/2785; 631/208; 631/61/514/1948; Anthropology; Biotin; Cancer; Chromosomes; Flow cytometry; Forensic science; Genetic analysis; Genomes; Humanities and Social Sciences; multidisciplinary; Nucleotide sequence; Science; Science (multidisciplinary); Streptavidin; Y chromosomes
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-27819-x

This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin–biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences.