Impact of root-associated strains of three Paraburkholderia species on primary and secondary metabolism of Brassica oleracea

• Published in: Scientific reports Vol. 11; no. 1; p. 2781
• Main Authors: Jeon, Je-Seung, Carreno-Quintero, Natalia, van Eekelen, Henriëtte D. L. M., De Vos, Ric C. H., Raaijmakers, Jos M., Etalo, Desalegn W.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 02-02-2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/158; 631/326; 631/449; 631/45; 631/553; 631/92; Brassica - metabolism; Brassica - microbiology; Burkholderiaceae - metabolism; Colonization; Cultivars; Energy demand; Flavonoids; Humanities and Social Sciences; Metabolism; Metabolites; multidisciplinary; Plant Diseases - prevention & control; Plant growth; Plant Leaves - metabolism; Plant metabolism; Plant Roots - metabolism; Plant Roots - microbiology; Science; Science (multidisciplinary); Secondary metabolites; Severity of Illness Index; Species; Strains (organisms); Sugar
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-82238-9

Several root-colonizing bacterial species can simultaneously promote plant growth and induce systemic resistance. How these rhizobacteria modulate plant metabolism to accommodate the carbon and energy demand from these two competing processes is largely unknown. Here, we show that strains of three
Paraburkholderia
species,
P. graminis
PHS1 (
Pbg
),
P. hospita
mHSR1 (
Pbh
), and
P. terricola
mHS1 (
Pbt
), upon colonization of the roots of two Broccoli cultivars led to cultivar-dependent increases in biomass, changes in primary and secondary metabolism and induced resistance against the bacterial leaf pathogen
Xanthomonas campestris.
Strains that promoted growth led to greater accumulation of soluble sugars in the shoot and particularly fructose levels showed an increase of up to 280-fold relative to the non-treated control plants. Similarly, a number of secondary metabolites constituting chemical and structural defense, including flavonoids, hydroxycinnamates, stilbenoids, coumarins and lignins, showed greater accumulation while other resource-competing metabolite pathways were depleted. High soluble sugar generation, efficient sugar utilization, and suppression or remobilization of resource-competing metabolites potentially contributed to curb the tradeoff between the carbon and energy demanding processes induced by
Paraburkholderia
-Broccoli interaction. Collectively, our results provide a comprehensive and integrated view of the temporal changes in plant metabolome associated with rhizobacteria-mediated plant growth promotion and induced resistance.