Diversity, Leishmania detection, and blood meal sources of sand flies from Iguatama, Minas Gerais, Brazil

• Published in: PloS one Vol. 19; no. 5; p. e0302567
• Main Authors: Dutra-Rêgo, Felipe, Binder, Camila, Capucci, Débora Cristina, Vaz, Talita Pereira, Andrade Filho, José Dilermando, Fontes, Gilberto, Gontijo, Célia Maria Ferreira
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 23-05-2024; Public Library of Science (PLoS)
• Subjects: Abdomen; Animals; Biodiversity; Biology and Life Sciences; Blood; Blood meals; Brazil - epidemiology; Chickens - parasitology; Deoxyribonucleic acid; DNA; Dogs; Dry season; Ecology and Environmental Sciences; Feeding Behavior; Females; Flies; Health aspects; Humans; Insect Vectors - parasitology; Leishmania; Leishmania - genetics; Leishmania - isolation & purification; Leishmaniasis; Leishmaniasis, Visceral - epidemiology; Leishmaniasis, Visceral - parasitology; Leishmaniasis, Visceral - transmission; Leishmaniasis, Visceral - veterinary; Light traps; Medicine and Health Sciences; Parasitic diseases; People and places; Protozoa; Psychodidae - parasitology; Public health; Rainy season; Rats; Regression analysis; Serology; Species diversity; Species richness; Vector-borne diseases; Vertebrates; Visceral leishmaniasis; Brazil; United States > US; California
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0302567

This study investigated the sand fly fauna of the municipality Iguatama, in the Midwest Region of Minas Gerais state, Brazil, including Leishmania infection rates and blood meal sources. Sand flies were collected during four periods over the course of a single year, encompassing both dry and rainy seasons, using CDC light traps placed in peridomiciles where dogs were seropositive for visceral leishmaniasis (VL). A total of 762 sand fly specimens, representing 12 species across seven genera, were collected. Lutzomyia longipalpis was the most abundant species, comprising 57.6% of the collected specimens, followed by Nyssomyia neivai (19.6%) and Nyssomyia whitmani (10.5%). Species richness and diversity varied among collection periods, with the highest diversity observed in January 2019. Molecular analysis detected Leishmania DNA in 12.5% of the sand fly specimens, with Le. infantum being the predominant species. Blood meal analysis revealed feeding on multiple vertebrate species, including humans, rats, dogs, and chickens. The presence of Leishmania DNA in sand flies, and the identification of human blood meals, highlight the potential role of these species in VL transmission. These findings underscore the importance of continued surveillance and control measures to prevent the spread of VL and reduce transmission risk in the region.