The Anaplasma marginale msp5 gene encodes a 19-kilodalton protein conserved in all recognized Anaplasma species

• Published in: Infection and Immunity Vol. 60; no. 12; pp. 5139 - 5144
• Main Authors: Visser, E.S. (Washington State University, Pullman, WA), McGuire, T.C, Palmer, G.H, Davis, W.C, Shkap, V, Pipano, E, Knowles, D.P. Jr
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-12-1992
• Subjects: Amino Acid Sequence; ANAPLASMA; Anaplasma - genetics; Anaplasma marginale; Animals; Antibodies, Monoclonal - immunology; ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; ANTIGENE; ANTIGENOS; BACTERIA; Bacterial Outer Membrane Proteins - genetics; Bacterial Outer Membrane Proteins - immunology; Bacteriology; Base Sequence; Biological and medical sciences; Cattle; CLONE; CLONES; Cloning, Molecular; DIAGNOSTIC; DIAGNOSTICO; Epitopes; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genes, Bacterial; IMMUNSERUM; MEMBRANAS CELULARES; MEMBRANE CELLULAIRE; Mice; Mice, Inbred BALB C; Microbiology; Molecular Sequence Data; Molecular Weight; Morphology, structure, chemical composition; NUCLEOTIDE; NUCLEOTIDOS; PROTEINAS UNICELULARES; PROTEINE MICROBIOLOGIQUE; RATON; RECOMBINACION; RECOMBINAISON; SOURIS; SUERO INMUNE; Membrane protein; Antigenic determinant; Gene product; Anaplasmataceae; Anaplasma marginale; Monoclonal antibody; External membrane; Rickettsiales; Conserved sequence; Antigenicity; Bacteria; Recombinant protein; Species
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/iai.60.12.5139-5144.1992

Immunization with Anaplasma marginale outer membranes induced immunity against clinical disease which correlated with antibody titer to outer membrane proteins, including a 19-kDa protein (N. Tebele, T. C. McGuire, and G. H. Palmer, Infect. Immun. 59:3199-3204, 1991). This 19-kDa protein, designated major surface protein 5 (MSP-5), was encoded by a single-copy 633-bp gene. The molecular mass of MSP-5, defined in immunoblots by binding to monoclonal antibody ANAF16C1, was conserved among all recognized species of Anaplasma: A. marginale, A. centrale, and A. ovis. Recombinant MSP-5, which absorbed the antibody reactivity of bovine immune serum to native MSP-5, was recognized by anti-A. marginale and anti-A. centrale immune sere in a competitive inhibition assay with monoclonal antibody ANAF16C1. The presence of antibody to the epitope defined by monoclonal antibody ANAF16C1 in all postinfection sera tested indicates that this epitope is a potential diagnostic antigen for use in identifying persistently infected cattle