rolC gene increases caffeoylquinic acid production in transformed artichoke cells

• Published in: Applied microbiology and biotechnology Vol. 98; no. 18; pp. 7773 - 7780
• Main Authors: Vereshchagina, Y. V, Bulgakov, V. P, Grigorchuk, V. P, Rybin, V. G, Veremeichik, G. N, Tchernoded, G. K, Gorpenchenko, T. Y, Koren, O. G, Phan, N. H. T, Minh, N. T, Chau, L. T, Zhuravlev, Y. N
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01-09-2014; Springer Berlin Heidelberg; Springer; Springer Nature B.V
• Subjects: Acid production; Acids; Alzheimer disease; Alzheimer's disease; Analysis; Artichokes; Asteraceae; Biology; Biomedical and Life Sciences; Biomedical research; Biosynthesis; Biotechnological Products and Process Engineering; Biotechnology; callus; cardoons; Cell culture; Chemical properties; chlorogenic acid; Cinnamates - metabolism; Cloning; Cynara cardunculus; Cynara scolymus - cytology; Cynara scolymus - metabolism; diabetes; Flowers & plants; Functional foods & nutraceuticals; Genes; Genetic aspects; high performance liquid chromatography; Life Sciences; Liquid chromatography; Mass spectrometry; metabolism; Metabolites; Microbial Genetics and Genomics; Microbiology; Plant Proteins - genetics; Plant Proteins - metabolism; Polyphenols; Quinic Acid - analogs & derivatives; Quinic Acid - metabolism; Science; Signal transduction; Soil sciences; Studies; Vegetables; Russia; Cynarin; Caffeoylquinic acids; gene; Plant cell culture
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-014-5869-2

Caffeoylquinic acids are found in artichokes, and they are currently considered important therapeutic or preventive agents for treating Alzheimer’s disease and diabetes. We transformed artichoke [the cultivated cardoon or Cynara cardunculus var. altilis DC (Asteraceae)] with the rolC gene, which is a known inducer of secondary metabolism. High-performance liquid chromatography with UV and high-resolution mass spectrometry (HPLC-UV-HRMS) revealed that the predominant metabolites synthesized in the transgenic calli were 1,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, and chlorogenic acid. The rolC-transformed calli contained 1.5 % caffeoylquinic acids by dry weight. The overall production of these metabolites was three times higher than that of the corresponding control calli. The enhancing effect of rolC remained stable over long-term cultivation.