New chiral reverse phase HPLC method for enantioselective analysis of ketorolac using chiral AGP column

New chiral reverse phase HPLC method for enantioselective analysis of ketorolac using chiral AGP column

A simple, specific, precise, sensitive and rapid reverse phase-HPLC method was developed for determination of ketorolac enantiomers, a potent nonnarcotic analgesic in pharmaceutical formulations. The method was developed on a chiral AGP column. Mobile phase was 0.1 M sodium phosphate buffer （pH 4.5）...

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Bibliographic Details
Published in:Journal of pharmaceutical analysis Vol. 2; no. 6; pp. 462 - 465
Main Authors: Dubey, Sunil K., Hemanth, Jangala, Venkatesh K., Chiranjeevi, Saha, R.N., Pasha, S.
Format: Journal Article
Language:English
Published: China Elsevier B.V 01-12-2012
Department of Pharmacy, Birla Institute of Technology & Science, Pilani, India%Institute of Genomics and Integrative Biology, Mall Road, Delhi, India
Xi'an Jiaotong University
Elsevier
Subjects:
AGP
Chiral AGP
R-Ketorolac
RP-HPLC
S-Ketorolac
反相高效液相色谱法
对映体
手性
流动相组成
紫外检测波长
选择性分析
酮咯酸
RP-HPLC
S-Ketorolac
Chiral AGP
R-Ketorolac
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Summary:A simple, specific, precise, sensitive and rapid reverse phase-HPLC method was developed for determination of ketorolac enantiomers, a potent nonnarcotic analgesic in pharmaceutical formulations. The method was developed on a chiral AGP column. Mobile phase was 0.1 M sodium phosphate buffer （pH 4.5）： lsopropanol （98：2, v/v）, at a flow rate of 1 mL/min with run time of 15 min. Ultraviolet detection was made at 322 nm. The linearity range was 0.02 10 μg/mL for each of the enantiomers. The mobile phase composition was systematically studied to find the optimum chromatographic conditions. Validation of the method under the conditions selected showed that it was selective and precise and that the detector response was linear function of ketorolac.
Bibliography:R-KetorolacS-Ketorolac;ChiralAGP;RP-HPLC
61-1484/R
A simple, specific, precise, sensitive and rapid reverse phase-HPLC method was developed for determination of ketorolac enantiomers, a potent nonnarcotic analgesic in pharmaceutical formulations. The method was developed on a chiral AGP column. Mobile phase was 0.1 M sodium phosphate buffer （pH 4.5）： lsopropanol （98：2, v/v）, at a flow rate of 1 mL/min with run time of 15 min. Ultraviolet detection was made at 322 nm. The linearity range was 0.02 10 μg/mL for each of the enantiomers. The mobile phase composition was systematically studied to find the optimum chromatographic conditions. Validation of the method under the conditions selected showed that it was selective and precise and that the detector response was linear function of ketorolac.
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SourceType-Scholarly Journals-1
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content type line 23
ISSN:2095-1779
2214-0883
DOI:10.1016/j.jpha.2012.07.006