Prevention of the Neurotoxicity of the Amyloid β Protein by Genipin

• Published in: Biological & pharmaceutical bulletin Vol. 24; no. 12; pp. 1454 - 1455
• Main Authors: YAMAZAKI, Matsumi, SAKURA, Naoki, CHIBA, Kenzo, MOHRI, Tetsuro
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 2001; Maruzen
• Subjects: Amyloid beta-Peptides - antagonists & inhibitors; Amyloid beta-Peptides - toxicity; amyloid β protein; Animals; Biological and medical sciences; Cells, Cultured; Cholagogues and Choleretics - pharmacology; Cholagogues and Choleretics - therapeutic use; Dose-Response Relationship, Drug; Embryo, Mammalian; General pharmacology; Genipin; Geniposide; hippocampal neuron; Hippocampus - cytology; Hippocampus - drug effects; Hippocampus - pathology; Iridoid Glycosides; Iridoids; Medical sciences; Nerve Growth Factor - pharmacology; Neurons - cytology; Neurons - drug effects; Neurons - pathology; Neuropharmacology; neuroprotection; Neuroprotective agent; neurotoxicity; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Pyrans - pharmacology; Rats; Iridoid; Gardenia; Pharmacognosy; Central nervous system; Neuroprotective agent; Antialzheimer agent; In vitro; Biological activity; Medicinal plant; Toxin; Neurotrophic factor; Neuron; β Amyloid protein; Dicotyledones; Angiospermae; Rubiaceae; Neurotoxin; Plant origin; Primary culture; Spermatophyta; Mechanism of action; Hippocampus; Brain (vertebrata)
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.24.1454

Genipin, which was shown in our previous investigation to have prominent neuritogenic activity in paraneurons such as PC12h cells, was studied to determine whether it could prevent the toxicity of Alzheimer’s amyloid β protein (Aβ) in cultured hippocampal neurons. Increased release of lactate dehydrogenase from hippocampal neurons after 2 d of Aβ25-35 administration was prevented dose dependently by the addition of genipin 20—40 μM. Morphological observations and trypan blue staining of cells confirmed the protection of hippocampal neurons from Aβ toxicity by genipin. Geniposide had less effect in preventing Aβ toxicity.